摘要
为制备高纯度β-葡聚糖,以元麦麸皮为原料,通过正交试验设计对酶解工艺进行优化,并进一步经柱层析纯化,制得β-葡聚糖纯品。确定了β-葡聚糖的优化酶解条件,即碱性蛋白酶的优化工艺是:料液比为1:15、酶用量为30mg/g、酶解时间为2 h;除去蛋白质的优化酶解工艺为:蛋白酶用量1 mg/mL,m(中性蛋白酶):m(胰蛋白酶)=1:2,酶解温度为45℃,酶解时间为2.5 h。该工艺制得的β-葡聚糖样品,利用凝胶色谱结合多角度激光散射仪测得其纯度为97.42%,相对分子质量为2.489×10^5D。
In order to prepare high purity β-glucan,by taking the naked barley bran as the raw material,the pure β-glucan product was prepared by optimization of enzymolysis technology through orthogonal test design and further purification by column chromatography.The optimum enzymolysis conditions were determined,that is,the optimum enzymolysis process of alkaline protease was:the ratio of material to liquid was 1:15 (W:V),consumption of alkaline protease was 30 mg/g and the enzymolysis time was 2 h.The optimum enzymolysis process for protein removal was:protease consumption was 1 mg/mL,ratio of neutral protease to trypsin was 1:2,the enzymolysis temperature was 45 ℃ and enzymolysis time was 2.5 h.The purity of the β-glucan sample obtained by this process was determined to be 97.42% by GPC/MALLS and the relative molecular mass of it was 2.489×10^5D.
作者
宋居易
刘建
魏亚凤
陈惠
Song Juyi;Liu Jian;Wei Yafeng;Chen Hui(Jiangsu Institute of Agriculture Sciences For Region Along The Yangtze River,Rugao 226541,China)
出处
《包装与食品机械》
CAS
北大核心
2019年第4期10-14,共5页
Packaging and Food Machinery
基金
江苏省农业科技自主创新资金资助[CX(14)2123]
2017年南通市应用基础研究项目[MS12017022-6]
关键词
元麦麸皮
Β-葡聚糖
提取
纯化
naked barley bran
β-glucan
extraction
purification
