摘要
背景:维生素D受体基因在调控机体钙磷以及骨骼代谢方面发挥关键作用,目前尚无其与胸腰段骨质疏松性椎体压缩骨折类型的关联研究报道。目的:利用限制性片段长度多态性聚合酶链反应技术,探讨维生素D受体基因多态性与胸腰段骨质疏松性椎体压缩骨折半定量分型的相关性。方法:研究符合广西中医药大学第一附属医院医学伦理委员会的相关制度,受试者均自愿签署知情同意书。随机纳入胸腰段骨质疏松性椎体压缩骨折患者60例,均拍摄全脊柱标准侧位X射线片,采用Genant半定量法进行骨折分型,并采集患者外周血的血清,通过DNA提取扩增、特异性酶切、凝胶电泳成像等方法,鉴定维生素D受体多态性位点基因型,并通过统计学方法分析其与胸腰段骨质疏松性椎体压缩骨折分型的关联性。结果与结论:①60例患者中检测到维生素D受体基因BB型0例,Bb型28例,bb型32例;②其中Ⅱ型(中度压缩)和Ⅲ型(重度压缩)椎体骨折Bb组占比87%,与bb组占比32%相比较,差异有显著性意义(χ~2=57.236,P<0.01);③结果说明,胸腰段骨质疏松性椎体压缩骨折患者维生素D受体不同基因型与骨折分型相互关联,Bb基因型患者发生中、重度压缩性椎体骨折的可能性高于bb基因型的患者。
BACKGROUND: Vitamin D receptor gene plays a key role in regulating calcium and phosphorus metabolism and bone metabolism. There are no reports on its association with osteoporotic vertebral compression fractures. OBJECTIVE: To investigate the relationship between vitamin D receptor gene polymorphism and semi-quantitative classification of thoracolumbar osteoporotic vertebral compression fractures by restriction fragment length polymorphism polymerase chain reaction. METHODS: The study was in accordance with the ethical requirements of the First Affiliated Hospital of Guangxi University of Chinese Medicine, and all participants signed the informed consents. Sixty patients with thoracolumbar osteoporotic vertebral compression fractures were randomly selected. The standard lateral X-ray films of the whole spine were taken. The Genant semi-quantitative method was used to classify the fracture types. The serum of peripheral blood was collected, and the genotype of vitamin D receptor polymorphism was identified by DNA amplification, specific enzyme digestion and gel electrophoresis imaging. The correlation between the polymorphisms of the vitamin D receptor and the thoracolumbar osteoporotic vertebral compression fractures classification was analyzed by statistical methods. RESULTS AND CONCLUSION:(1) Vitamin D receptor gene BB type was detected in 0 case, Bb type in 28 cases and BB type in 32 cases.(2) Type II(moderate compression) and type III(severe compression) Bb vertebral fractures accounted for 87%, compared with Bb group accounted for 32%, the difference was significant(χ~2=57.236, P < 0.01).(3) These results indicate that different genotypes of vitamin D receptor in thoracolumbar osteoporotic vertebral compression fractures patients are correlated with fracture types. The risk of moderate to severe compressive vertebral fractures in patients with Bb genotype is higher than that in patients with bb genotype.
作者
卢秋平
桂裕昌
许建文
覃海飚
饶远森
Lu Qiuping;Gui Yuchang;Xu Jianwen;Qin Haibiao;Rao Yuansen(First Clinical Medical College of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China;Department of Rehabilitation Medicine, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China;Department of Orthopedics, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第35期5577-5581,共5页
Chinese Journal of Tissue Engineering Research
基金
广西科学研究与技术开发计划课题(桂科攻10124001B-38)，项目负责人：许建文
广西壮族自治区卫生和计划生育委员会课题(Z2014119)，项目负责人：覃海彪
广西壮族自治区康复临床重点专科建设项目(桂卫医发[2018]6号)，项目负责人：许建文~~
关键词
维生素D受体
基因型
多态性
椎体骨折
压缩性骨折
骨质疏松
骨折类型
定量分型
vitamin D receptor
genotype
gene polymorphism
vertebral fracture
compressive fracture
osteoporosis
fracture classification
quantitative classification
