摘要
背景:研究发现,川芎嗪能有效保护失用状态下大鼠骨骼肌细胞、对抗失神经大鼠骨骼肌萎缩。目的:探究川芎嗪对大鼠静态姿势负荷后不同时相自由基及酶代谢的影响。方法:实验方案经华中科技大学附属同济医院实验动物实验伦理委员会批准。36只SPF级雄性SD大鼠随机被分为对照组、负荷模型组、川芎嗪组。实验开始前0.5h,对照组、负荷模型组大鼠灌胃2mL溶媒,川芎嗪组大鼠灌胃川芎嗪溶液2mL。实验过程中对照组大鼠不施加任何负荷;负荷模型组、川芎嗪组大鼠以夹板固定四肢持续实施静态姿势负荷,且躯干部分固定在预先制作好的木盒中保持伸直状态10h,建立静态姿势负荷大鼠模型。检测川芎嗪干预不同时相(2,5,8和10h)大鼠血清丙二醛水平、血清乳酸脱氢酶活性及比目鱼肌钙ATP酶(Ca2+-ATPase)活力变化。结果与结论:①对照组大鼠血清丙二醛浓度、血清乳酸脱氢酶活性及比目鱼肌Ca2+-ATPase活力不随时间变化而改变;与负荷模型组2h比较,同组5,8和10h的丙二醛浓度、血清乳酸脱氢酶活性均显著升高,Ca2+-ATPase活力均显著降低(P<0.05或P<0.01);与川芎嗪组2h比较,同组5,8和10h的丙二醛浓度、血清乳酸脱氢酶活性均显著降低,Ca2+-ATPase活力均显著升高(P<0.05或P<0.01);与对照组同时相比较,负荷模型组大鼠血清中丙二醛自由基水平、乳酸脱氢酶活性均显著增加,Ca2+-ATPase活力显著降低(P<0.01);与负荷模型组同时相比较(2h除外),川芎嗪组大鼠丙二醛自由基水平、乳酸脱氢酶活性均显著降低,Ca2+-ATPase活力显著增加(P<0.01);②结果说明,川芎嗪能显著降低不同时相静态姿势负荷引起的血清丙二醛浓度及血清乳酸脱氢酶活性,显著升高目鱼肌Ca2+-ATPase活力,以达到缓解长时间静态姿势负荷引起的机体损伤。
BACKGROUND: Ligustrazine has been shown to protect the rat skeletal muscle under state of disuse, and protect against skeletal muscle atrophy in denervated rats. OBJECTIVE: To investigate the effects of ligustrazine on different phases of free radicals and enzyme metabolism after static postural load in rats. METHODS: The study was approved by the Laboratory Animal Ethical Committee of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. Thirty-six SPF male Sprague-Dawley rats were randomized into control, model and ligustrazine groups. At 0.5 hour before experiment, the rats in the control and model groups were given 2 mL solvent via gavage, and ligustrazine group was given 2 mL ligustrazine solution. During the experiment, the rats in the control group received no load. The rats in the model and ligustrazine groups were fixed with the splint to maintain the static posture load, and the trunk part was fixed in the pre-made wooden box to maintain the straight state for 10 hours, to establish a static posture load rat model. The serum level of malonaldehyde, serum lactic dehydrogenase activity and Ca2+-ATPase activity in the soleus at different phases(2, 5, 8 and 10 hours) were detected. RESULTS AND CONCLUSION:(1) The serum level of malonaldehyde, serum lactic dehydrogenase activity and Ca2+-ATPase activity in the soleus did not change with time in the control group. Compared with 2 hours, in the model group, the serum level of malonaldehyde and serum lactic dehydrogenase activity were increased, and the Ca2+-ATPase activity was significantly decreased at 5, 8 and 10 hours(P < 0.05 or P < 0.01). Compared with 2 hours, in the ligustrazine group, the serum level of malonaldehyde and serum lactic dehydrogenase activity were decreased, and the Ca2+-ATPase activity was significantly increased at 5, 8 and 10 hours(P < 0.05 or P < 0.01). Compared with the control group at the same phase, the serum level of malonaldehyde and serum lactic dehydrogenase activity in the model group were increased, and the Ca2+-ATPase activity was significantly decreased(P < 0.01). Compared with the model group at the same phase(except 2 hours), the serum level of malonaldehyde and serum lactic dehydrogenase activity were significantly decreased in the ligustrazine group, and the Ca2+-ATPase activity was significantly increased(P < 0.01).(2) These results indicate that ligustrazine can significantly reduce the serum malonaldehyde content and serum lactic dehydrogenase activity caused by static postural load at different phases, and significantly increase the Ca2+-ATPase activity in the soleus, so as to alleviate the body injury caused by long-term static postural load.
作者
肖凯
周江红
高鑫峰
赵晶晶
方真华
杨林
Xiao Kai;Zhou Jianghong;Gao Xinfeng;Zhao Jingjing;Fang Zhenhua;Yang Lin(Wuhan Fourth Hospital & Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China;Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第35期5600-5604,共5页
Chinese Journal of Tissue Engineering Research
基金
湖北省卫生计生委自然科学基金面上项目(2016CFB367)，项目负责人：肖凯~~
