摘要
为探究多形拟杆菌对小鼠急性铅毒性的影响,在急性铅暴露期间灌胃多形拟杆菌FTJS-8-K,测定了小鼠组织、粪便及血液中铅含量、肝肾功能损伤程度、肠屏障功能及肠道菌群的变化.结果表明,补充多形拟杆菌FTJS-8-K,可促进铅随粪便排出,减少组织铅蓄积,恢复了肝肾组织中丙二醛、谷胱甘肽水平,降低了氧化应激损伤的程度,提高了结肠与小肠中ZO-1、Occludin mRNA的表达,改善了由于急性铅暴露引起的肠道通透性增加,促使肠道中短链脂肪酸(short chain fatty acids,SCFAs)含量增加.同时,多形拟杆菌FTJS-8-K可以改善急性铅暴露引起的肠道菌群紊乱,降低肠杆菌、假单胞菌属等条件致病菌的丰度,增加了瘤胃球菌、Rikenellaceae__unclassi-fied、S24-7__unclassified等的丰度.综上,多形拟杆菌FTJS-8-K可以显著缓解铅暴露引起的机体损伤,为今后通过膳食干预策略拮抗铅毒性提供了参考依据.
This study evaluated the modulating effects of Bacteroides thetaiotaomicron ( B.thetaiotaomicron ) on acute lead (Pb) toxicity. B. thetaiotaomicron FTJS-8-K was orally administrated to mice during acute Pb exposure followed by measurement of the contents of Pb in tissues,feces and blood,the function of liver,kidney and intestinal barrier,and analysis of the gut microbiota. The results showed that B. thetaiotaomicron FTJS-8-K could promote the excretion of Pb in feces and reduce Pb accumulation in tissues,which consequently reduced oxidative stress by restoring the levels of malondialdehyde and glutathione in the liver and kidney. Besides,the expressions of ZO-1 and Occludin increased in colon and small intestine,the intestinal permeability improved and the level of short chain fatty acids in intestinal tract increased. In addition,strain FTJS-8-K significantly improved gut bacterial community,as the abundances of Enterobacter sp. and Pseudomonas sp . decreased,while the abundances of Ruminococcus ,Rikenellaceae unclassified and S24-7 unclassified increased. Therefore,B. thetaiotaomicron FTJS-8-K can significantly alleviate damages to body caused by Pb,which indicates the potentials of reducing heavy metal toxicity by diets.
作者
屈定武
翟齐啸
于雷雷
田丰伟
赵建新
张灏
陈卫
QU Dingwu;ZHAI Qixiao;YU Leilei;TIAN Fengwei;ZHAO Jianxin;ZHANG Hao;CHEN Wei(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2019年第16期54-62,共9页
Food and Fermentation Industries
基金
国家自然科学基金重点项目(31530056)
关键词
多形拟杆菌
益生菌
急性铅毒性
肠道功能
肠道菌群
Bacteroides thetaiotaomicron
probiotics
acute lead toxicity
intestinal function
gut microbiota
