摘要
目的研究川芎、冰片配伍对全脑缺血大鼠海马和下丘脑神经元的保护机制。方法将全脑缺血大鼠分为假手术组、模型组、川芎组(1.0 g·kg^-1)、冰片组(0.16 g·kg^-1)、川芎+冰片组。利用GC-MS方法检测海马/下丘脑的透析液中谷氨酸(glutamate,Glu)、甘氨酸(glycine,Gly)和γ-氨基丁酸(γ-aminobutyric acid,GABA)含量;激光共聚焦技术检测脑片中钙离子含量;TUNEL和电镜技术检测这两个脑区凋亡百分率和超微结构。结果川芎和冰片单用均可减少钙超载,缓解超微结构的改变,联合应用效果更佳。另外,川芎单用可减少两脑区的神经元凋亡指数、升高GABA。冰片仅降低两脑区的Glu,而它们的配伍还可进一步升高下丘脑Gly含量,也表现较好的协同关系。结论川芎和冰片配伍在缓解神经元兴奋毒、钙超载和超微结构异常方面具有较好的协同效应,但抗凋亡方面未见明显协同。
Aim To research the synergic effect of Chuanxiong Rhizoma(CR) and borneol(BO) on protecting hippocampus and hypothalamus of the rats from global cerebral ischemia reperfusion(GCIR) injury.Methods GCIR rats were divided into five groups, i.e. sham, model, CR(1.0 g·kg^-1 ), BO(0.16 g·kg ^-1 ) and CR+BO groups. The contents of Glu, Gly and GABA in brain microdialysis solution were detected by GC-MS.[Ca^2+] i of the two regions were measured using a confocal microscope. The apoptotic rate and ultrastructure were obtained by TUNEL stain and transmission electron microscopy, respectively. Results The monotherapy of CR or BO attenuated Ca^2+ overload and improved ultrastructure in both areas. Their synergy showed a better effect. CR alone reduced the apoptotic rate, increased the levels of GABA in both brain areas, while BO only decreased that of Glu. The combination even enhanced the content of Gly in hypothalamus. Conclusions Obvious synergic effects between CR and BO exist in attenuating neurocellular excitotoxicity and calcium overload, while the anti-apoptotic effect might be excluded.
作者
喻斌
阮鸣
许立
刘圣金
许惠琴
沈祥春
YU Bin;RUAN Ming;XU Li;LIU Sheng-jin;XU Hui-qin;SHENG Xiang-chun(Province and Ministry Cooperation Lab of Pharmacology of Chinese Medicine/Jiangsu Key Lab for Pharmacology and Safety Evaluation of Chinese Materia Medica,Nanjing University of Chinese Medicine, Nanjing 210023, China;School of Food Technology, Nanjing Xiaozhuang University, Nanjing 211117,China;the Key Lab of Optimal Utilization of Natural Medicinal Resources, Guizhou Medical University, Guiyang 550025,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2019年第9期1302-1308,共7页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81573713)
江苏省自然科学基金资助项目(No BK20151564)
江苏省青蓝工程(2017)
贵州省科技厅项目(黔科合[2016]支撑2826)
贵州省高等学校科技创新团队(No 2014-31)
贵州省科学技术创新团队(No 2015-4025)
高层次创新人才(No 2015-4029)
关键词
川芎
冰片
配伍
全脑缺血/再灌注
兴奋毒
钙超载
Chuanxiong Rhizoma
borneol
combining therapy
GCIR
excitotoxicity
calcium overload
