摘要
【目的】探究新白8号枇杷香气成分及内标物的最适浓度,以期为白肉枇杷香气成分的研究及内标选择提供依据。【方法】以白肉枇杷新白8号果实为试材,加入4种不同浓度等体积的环己酮溶液(947、94.7、9.47、0.947μg·μL^-1)为内标,采用HS-SPME-GC/MS技术,对试材的香气成分进行定性和定量分析。【结果】新白8号枇杷果肉中共检测出10类43种香气成分,以醛类、烷烃、芳香烃、酮类和醇类为主,其中相对含量较高的物质有己醛、(E)-2-己烯醛、正丁基环己烷、2-庚酮、正戊基环己烷、(E)-2-己烯-1-醇,苯乙烯等。4种环己酮浓度的样品分别检测出有效香气化合物为30、39、41和32种,有效峰面积占总峰面积比分别为38.20%、28.49%、42.70%和39.51%;环己酮峰面积比分别为47.38%、58.55%、36.37%和25.86%。加入9.47μg·μL^-1环己酮的样品检测出的香气物质种类最多、有效峰面积比最高、环己酮及各香气成分峰面积占比适宜。【结论】新白8号香气成分丰富多样,9.47μg·μL^-1环己酮为枇杷果实香气成分固相微萃取的适宜内标浓度。
【Objective】To determine the aromatic composition of loquat fruit and cyclohexanone concentration for the HS-SPME-GC/MS internal standard method.【Methods】The pulp of Xinbai 8 fruits was added with a cyclohexanone solution in the concentration of 947,94.7,9.47,0.947 μg·μL^-1 at a same volume as the internal standard for HS-SPME-GC/MS analysis.【Results】 The analysis detected 10 classifications of chemicals including aldehydes,alkanes,dutrex,ketones and alcohols.Hexanal,(E)-2-hexenal,n-butyl cyclohexane,2-heptanone,n-pentyl cyclohexane,(E)-2-hexene-1-alcohol,and styrene were the dominating volatiles among the 43 compounds identified.In the cyclohexanone-added samples,the decreasing concentration produced 31,40,42 and 33 aromatic substances with the effective peak area ratios of 38.20%,28.49%,42.70% and 39.52%,and the peak area ratios of 38.20%,58.55%,36.37% and 25.86%,respectively.A maximum number of aromatics was found in the sample that was added with 9.47 μg·μL^-1 cyclohexanone which also had the highest effective peak area ratio and optimum peak area radio.【Conclusion】The aromatics in the fruits of Xinbai 8 were diverse,and the cyclohexanone concentration at 9.47 μg·μL^-1 was the choice internal standard for the analytical.
作者
张桂彬
安宁
朱立辉
蒋际谋
胡文舜
ZHANG Gui-bin;AN Ning;ZHU Li-hui;JIANG Ji-mou;HU Wen-shun(Horticultural College,Fujian Agricultural and Forestry University,Fuzhou,Fujian 350002,China;Institute of Fruit Trees,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350002,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2019年第6期660-667,共8页
Fujian Journal of Agricultural Sciences
基金
福建省财政专项——福建省农业科学院科技创新团队建设项目(STIT2017-1-4)
福建省科技星火计划项目(217S0009)
