摘要
淋球菌感染所致淋病是最常见的性传播疾病之一。随着抗菌药物的不合理使用以及淋球菌的不断变异,淋球菌的耐药问题在全球范围内已经十分严重。目前,伴有多重耐药的菌株快速增多,临床亟需淋球菌药物敏感性检测快速准确的方法,以为淋病的有效防治提供支撑。近年来,核酸检测方法因基耗时短,自动化等优点为淋球菌及耐药监测领域提供了新的手段和机遇,今后有望取代培养法成为淋球菌药物敏感性检测的首选方法。其中,全基因组测序技术可提供全面的耐药信息,可进行进化分析,在耐药监测领域显示出了更高效的性能以及广阔的应用前景。但是,核酸检测方法在淋球菌耐药检测方面仍面临着交叉反应、依赖设备等重重挑战。总之,核酸检测方法在淋球菌耐药监测相关领域已经取得一定的进展,但仍面临重大挑战,我们应该克服现有的挑战并且不断开发可用于淋球菌药物敏感性检测的新型核酸检测技术。
Gonorrhea,caused by gonococcal infection,is one of the most common sexually transmitted diseases.With the iiTational use of antibiotics and the evolution of Neisseria gonorrhoeae,the antimicrobial resistance (AMR) of Neisseria gonorrhoeae has become a serious problem worldwide.At present,as reports of gonococcal strains exhibiting multidrug resistance (MDR) are increasing rapidly,the rapid and accurate methods for gonococcal sensitivity detection are in urgent demand to provide support for effective prevention and treatment of gonorrhea.In recent years,nucleic acid detection has provided new method and opportunities for gonococcal sensitivity detection, because of the advantages of short time consumption and automation application,and may gradually replace the traditional culture methods,making it as the first choice for sensitivity detection of Neisseria gonorrhoeae.Among them,the technology of whole genome sequencing (WGS) can provide comprehensive AMR information and evolutionary analyses simultaneously,and gradually show more efficient performance and broad application prospects in the field of AMR monitoring.However,nucleic acid detection methods still face challenges such as cross-reaction and equipment dependence.In summary,nucleic acid detection methods have made great progresses in gonococcal AMR surveillance,substantial challenges still exist.We should overcome the existing challenges and continue to develop nucleic acid detection technologies for gonococcal sensitivity detection monitoring.
作者
李雅梅
彭俊平
Li Yamei;Peng Junping(NHC Key Laboratory of Systems Biology of Pathogens,Institute of Pathogen Biology,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100176,China)
出处
《国际流行病学传染病学杂志》
CAS
2019年第4期268-272,共5页
International Journal of Epidemiology and Infectious Disease
关键词
奈瑟球菌
淋病
耐药
核酸检测技术
全基因组测序技术
Neisseria gonorrhoeae
Antimicrobial resistance
Nucleic acid detection technology
Wholegenome sequencing technology
