摘要
目的探讨他克莫司对HaCaT细胞分泌趋化因子的影响。方法收集2017年6—10月就诊于浙江省义乌市皮肤病医院皮肤科和杭州市第三人民医院皮肤科的50例白癜风患者,取其白斑部位以及正常部位表皮疱液,用Luminex分析白癜风患者局部趋化因子水平,检测与白癜风密切相关的趋化因子。用Western印迹法分析他克莫司对HaCaT细胞趋化因子分泌以及相关信号通路的影响。结果通过疱液Luminex分析,发现白癜风白斑部位CXCL9与CXCL10水平[(7 482±628) pg/ml、(3 770±536) pg/ml)]显著高于正常部位疱液水平[(4 203±459) pg/ml、(1 941±468) pg/ml)],两者比较,差异有统计学意义(P<0.05)。γ干扰素可显著刺激HaCaT细胞CXCL9与CXCL10表达以及JAK1,STAT1磷酸化。20 mg他克莫司预处理HaCaT细胞后,可显著抑制γ干扰素刺激后HaCaT细胞CXCL9、CXCLl0表达以及JAK1,STAT1磷酸化。结论他克莫司可显著降低HaCaT细胞在应激状态下,CXCL9与CXCL10表达以及相关信号通路的激活,进而起到治疗白癜风的作用。
Objective To analyze the abnormalities of local chemokines in patients with vitiligo and to explore the effect of tacrolimus on the secretion of chemokines in keratinocytes. Methods Blister fluids of 50 patients with vitiligo were collected, including lesion areas and normal areas. Luminex was used to analyze the concentration of local chemokines in patients with vitiligo to determine whether the chemokines were closely related to vitiligo. The effect of tacrolimus on chemokine secretion of was analyzed by Western blot in HaCaT cells. Results By Luminex analysis of blister fluid, it was found that CXCL9 and CXCL10 were significantly higher in the leukoplakia of vitiligo, and there was a significant difference, compared with the blister fluid in the normal site (P<0.01). IFN-γ significantly stimulated the keratinocyte cell line HaCat to express CXCL9 and CXCL10. After pretreatment of HaCaT cells with 20 mg tacrolimus, the expression of CXCL9 and CXCL10 was significantly decreased, compared with the blank control (P<0.01). Conclusions The leukoplakia chemokines CXCL9 and CXCL10 are highly expressed in vitiligo patients. The tacrolimus can significantly reduce the expression of CXCL9 and CXCL10 in keratinocytes under stress, and it therefore plays a therapeutic role in vitiligo.
作者
徐永平
周妙妮
金嵘
许文
许爱娥
Xu Yongping;Zhou Miaoni;Jin Rong;Xu Wen(Department of Dermatology,Yiwu Dermatology Hospital,Yiwu 322000,China;Department of Dermatology,Hangzhou Third People's Hospita,Hangzhou 310009,China)
出处
《中华医学美学美容杂志》
2019年第4期322-325,共4页
Chinese Journal of Medical Aesthetics and Cosmetology
基金
国家自然科学基金(81773335、81602755)
浙江省义乌市一般科研计划项目(17-1-30).
