摘要
目的:探讨急性白血病患者红细胞生成素(EPO)及红细胞生成素受体(EPOR)的表达及其临床意义。方法:应用ELISA检测骨髓血浆中EPO和EPOR水平;RT-RCR法检测EPO和EPORmRNA表达水平;Westernblot法检测EPO和EPOR蛋白表达水平。结果:ALL及AML组骨髓血浆中EPO蛋白水平明显高于对照组(P<0.05);ALL及AML组骨髓血浆中EPOR蛋白水平明显低于对照组(P<0.05)。在ALL及AML组骨髓中EPO及EPOR mRNA水平均高于对照组(P<0.05);在ALL、AML高危组EPO及EPORmRNA水平较中危、低危及对照组均明显增高(P<0.05)。在ALL及AML组骨髓中EPO、EPOR蛋白水平均高于对照组(P<0.05)。ALL及AML组EPO、EPORmRNA水平与血红蛋白及红细胞无相关性(P>0.05)。结论:ALL及AML患者存在有EPO、EPOR高表达。EPO、EPOR表达水平与ALL及AML的危险度有关,在高危患者表达水平更高。EPO、EPOR水平与血红蛋白及红细胞之间无相关性。
Objective:To investigate the expression of erythropoietin(EPO)and erythropoietin receptor(EPOR)in patients with acute leukemia(AL)and its clinical significance.Methods:The levels of EPO and EPOR in plasma were determined by ELISA kit.mRNA expression levels of EPO and EPOR were determined by RT-RCR.The protein expression levels of EPO and EPOR were detected by Western blot.Results:The EPO protein levels in marrow plasma of ALL and AML group were significantly higher than those in the control group(P<0.05),EPOR protein levels in ALL and AML group were significantly lower than those in the control group(P<0.05).The mRNA levels of EPO and EPOR in ALL and AML groups were significantly higher than those in the control group(P<0.05).The mRNA levels of EPO and EPOR in the high risk ALL and AML groups were significantly higher than those in the medium,low risk group and the control group(P<0.05).The protein expression levels of EPO and EPOR in ALL and AML groups were significantly higher than that in control group(P<0.05).The mRNA levels of EPO and EPOR in ALL and AML groups did not correlate with hemoglobin level and erythrocyte count(P>0.05).Conclusion:The expressions of EPO and EPOR is higher in ALL and AML patients.The expression levels of EPO and EPOR relate with the risk of ALL and AML.High risk patients have higher expression levels of EPO and EPOR,however,the expression levels of EPO and EPOR do not correlate with hemoglobin level and erythrocyte counting.
作者
田润梅
黄成双
苏琼
史良
罗茜
陈艳
TIAN Run-Mei;HUANG Cheng-Shuang;SU Qiong;SHI Liang;LUO Xi;CHEN Yan(Department of Pediatrics,Affiliated Hospital of Zunyi Medical University,Zunyi 563003,Guizhou Province,China)
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2019年第4期1026-1032,共7页
Journal of Experimental Hematology
基金
国家自然科学基金(81641008)
