摘要
目的:制备白藜芦醇(RES)-羟丙基-β-环糊精(HP-β-CD)-壳聚糖缓释微球(RES-HP-β-CD-Chitosan),并进行表征。方法:按质量比1∶7∶0.25称取RES原料药、HP-β-CD和壳聚糖,先采用溶剂法制备RES-HP-β-CD包合物,在此基础上加入壳聚糖,采用喷雾干燥法制得RES-HP-β-CD-Chitosan。采用光学显微镜观察所制缓释微球的粒径。采用X射线衍射法、差式扫描量热法、红外光谱法、扫描电子显微镜对所制RES-HP-β-CD-Chitosan进行表征。采用紫外分光光度法测定所制缓释微球中RES的含量,计算载药量和包封率。结果:所制RES-HP-β-CD-Chitosan的粒径为(2.23±0.35)μm(n=300),表征结果显示,RES-HP-β-CDChitosan呈球形,微球表面出现收缩褶皱,RES被包合于羟丙基-β-环糊精中,以分子状态或无定型状态存在。RES-HP-β-CD-Chitosan的载药量为11.67%(n=3)、包封率为96.27%(n=3)。结论:成功制得RES-HP-β-CD-Chitosan。
OBJECTIVE:To prepare Resveratrol-hydroxypropyl-β-cyclodextrin-chitosan sustained-release pellets (RES-HP-β- CD-Chitosan),and to characterize it. METHODS:Resveratrol raw material,HP-β-cyclodextrin and chitosan were collected with ratio of 1 ∶ 7 ∶ 0.25. Resveratrol-HP-β-cyclodextrin inclusion compound were prepared by solvent method,and then added into chitosan,RES-HP-β-CD-Chitosan were prepared by spray drying method. Particle size of prepared sustained-released pellets were observed by optical microscope. X-ray,DSC,IR and SEM were used to characterize RES-HP-β-CD-Chitosan. The contents of resveratrol in prepared sustained-released pellets were determined by UV spectrum,and drug-loading amount and encapsulation efficiency were calculated. RESULTS:Particle size of prepared RES-HP-β-CD-Chitosan was (2.23 ± 0.35)μm(n=300). Characterization results show that RES-HP-β-CD-Chitosan was spherical in shape;shrinkage was found on the surface of microspheres,and resveratrol was included in HP-β-cyclodextrin in molecule or amorphous state. Drug-loading amount of prepared RES-HP-β-CD-Chitosan was 11.67%(n=3),encapsulation efficiency was 96.27%(n=3). CONCLUSIONS:RES-HP-β-CDChitosan is prepared successfully.
作者
张纯刚
于琛琛
周旖璇
尹丽
程岚
康廷国
韩岚
ZHANG Chungang;YU Chenchen;ZHOU Yixuan;YIN Li;CHENG Lan;KANG Tingguo;HAN Lan(School of Pharmacy,Liaoning University of Traditional Chinese Medicine,Liaoning Dalian 116620,China;School of Pharmacy,Anhui University of Traditional Chinese Medicine,Hefei 230012,China)
出处
《中国药房》
CAS
北大核心
2019年第17期2322-2326,共5页
China Pharmacy
基金
国家自然科学基金资助项目（No.81503257）
国家重点研发计划课题（No.2018YFC1706903）
辽宁省博士科研启动基金计划立项项目（No.201501098）
关键词
白藜芦醇
羟丙基-Β-环糊精
壳聚糖
制备
表征
Resveratrol
Hydroxypropyl-β-cyclodextrin
Chitosan
Preparation
Characterization
