摘要
目的探讨神经胶质瘤患者血浆外泌体长链非编码RNA LINC00511(lncRNA LINC00511)的表达水平及临床意义。方法选取2012 01-2016 04郑州人民医院收治的82例神经胶质瘤患者为研究对象,选取同期80例体检健康者为对照组,采用实时荧光定量PCR(qRT PCR)检测所有受试者血浆外泌体lncRNA LINC00511水平,观察受试者血浆外泌体lncRNA LINC00511表达情况,并探讨其与临床病理参数和预后的关系。结果 WHO Ⅲ~Ⅳ级、Ⅰ~Ⅱ级神经胶质瘤患者血浆外泌体lncRNA LINC00511水平明显高于对照组( P <0.05),Ⅲ~Ⅳ级患者高于Ⅰ~Ⅱ级患者( P <0.05);lncRNA LINC00511与患者WHO分级、KPS评分相关( P <0.05);lncRNA LINC00511高表达组生存率为34.09%,明显低于低表达组的65.79%( P <0.05),高表达组中位数生存时间为(42.00±3.60)个月,明显低于低表达组的(51.00±6.07)个月( P <0.05);多因素COX比例分析显示,高表达水平的lncRNA LINC00511、高WHO分级及KPS评分≤80分是神经胶质瘤患者预后不良的危险因素。结论 lncRNA LINC00511在神经胶质瘤患者血浆外泌体中表达上调,并与临床病理特征及预后密切相关。
Objective To investigate the expression and clinical significance of long non coding RNA LINC00511 (lncRNA LINC00511) in plasma exosomes of glioma patients. Methods 82 cases of glioma patients admitted to our hospital from January 2012 to April 2016 were selected as the research objects,and 80 healthy persons in the same period were selected as the control group.The level of lncRNA LINC00511 in plasma exosomes of all subjects was detected by real time fluorescence quantitative PCR (qRT PCR),the expression of lncRNA LINC00511 in plasma exosome of the subjects was observed,and its relationships with clinicopathological parameters and prognosis were analyzed. Results The level of plasma exosome lncRNA LINC00511 in patients with WHO grade ⅢⅣ and grade ⅠⅡ glioma was significantly higher than that in control group ( P <0.05),and that in patients with WHO grade ⅢⅣ was higher than that in patients with grade ⅠⅡ( P <0.05).LncRNA LINC00511 was correlated with WHO grade and KPS score ( P <0.05).The survival rate of the high expression group of lncRNA LINC00511 was 34.09%,which was significantly lower than 65.79% of the low expression group ( P <0.05),and the median survival time of the high expression group was (42.00±3.60) months,which was significantly lower than (51.00±6.07) months of the low expression group ( P <0.05).Multivariate COX analysis showed that high expression of lncRNA LINC00511,high WHO grade and KPS score<80 were risk factors for poor prognosis of glioma patients. Conclusion The expression of lncRNA LINC00511 in plasma exosomes of glioma patients is up regulated,which is closely related to clinicopathological features and prognosis.
作者
王博
王亚周
王凯
靳腾龙
崔丙周
贺艳阳
WANG Bo;WANG Yazhou;WANG Kai;JIN Tenglong;CUI Bingzhou;HE Yanyang(Department of Neurosurgery,Zhengzhou People's Hospital,Zhengzhou 450003,China)
出处
《中国实用神经疾病杂志》
2019年第14期1548-1553,共6页
Chinese Journal of Practical Nervous Diseases
基金
河南省科技基金项目(编号:182102310237)
