摘要
偏振成像可用于表征各向异性肌肉组织,但是常规偏振成像方法只能给出定性的结构形态,无法对肌肉发育过程进行定量的比较。构建了一套定量偏振成像系统,采用两个互相垂直的偏振片同步旋转得到一系列图像,经过图像处理获取斑马鱼每个肌节定量的相位延迟量。该定量偏振图像与光源亮度、曝光时间等无关,可以用来比较不同时间拍摄的斑马鱼肌肉形态和分布。研究发现野生型和肌肉受损型斑马鱼肌肉生长变化过程不同。
Polarization imaging can be used to characterize anisotropic muscle tissue,but the conventional methods can only give the qualitative structure and morphology of muscle development rather than quantitative comparison.In this paper,a quantitative polarization imaging system is constructed.Two perpendicular polarizers are rotated synchronously to get a set of images,and then the quantitative phase retardance of each zebrafish sarcomere is calculated through image processing.This quantitative polarization image is independent of the brightness of light source,the exposure time,etc.,hence can be used to compare the morphology and distribution of zebrafish muscle taken at different time.The results revealed differences in the process of muscle growth between wild-type and muscle-damaged zebrafish.
作者
朱雨
杨光
李思黾
王林波
金鑫
梁永
赵唯初
李辉
Zhu Yu;Yang Guang;Li Simin;Wang Linbo;Jin Xin;Liang Yong;Zhao Weichu;Li Hui(Department of Precision Machinery and Precision Instrumentation,University of Science and Technology of China,Hefei,Anhui 230026,China;Jiangsu Key Laboratory of Medical Optics,Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou,Jiarigsu 215163,China)
出处
《光学学报》
EI
CAS
CSCD
北大核心
2019年第8期133-139,共7页
Acta Optica Sinica
基金
国家重点研发计划(2017YFC0110100)
国家自然科学基金(61805272)
中国科学院科研装备研制项目(YZ201646)
王宽诚教育基金
关键词
医用光学
定量偏振成像
相位延迟量
斑马鱼
肌肉生长
medical optics
quantitative polarization imaging
phase retardance
zebrafish
muscle growth
