摘要
目的建立LDHW-FFZ1-WuYH层析柱提取吗啡的方法,以及探讨氢氯噻嗪对吗啡中毒家兔体内吗啡代谢动力学的影响。方法选择家兔6只,随机分为实验组(吗啡+氢氯噻嗪)和对照组(吗啡),每组3只。实验组经耳缘静脉注射盐酸吗啡生理盐水溶液,随后采用氢氯噻嗪生理盐水经口灌胃,30min后分别于1、2、3、4、5、6、7、8h经颈总动脉各取1mL血液冷冻保存待检。对照组仅采用经耳缘静脉注射盐酸吗啡生理盐水溶液,30min后分别于1、2、3、4、5、6、7、8h经颈总动脉各取1mL血液冷冻保存待检。采用LDHW-FFZ1-WuYH层析柱进行前处理、气相色谱氮磷检测器(GC/NPD)定量检测吗啡水平。结果LDHW-FFZ1-WuYH层析柱提取回收率高。对照组在3、4、5、8h的吗啡水平均高于实验组,差异有统计学意义(P<0.05)。结论建立了LDHW-FFZ1-WuYH层析柱提取吗啡的方法,该法成本低、方便快捷、回收率高、杂质水平低,可应用于吗啡中毒致死案件检材的前处理。利尿剂氢氯噻嗪可加速吗啡在体内的代谢速度,缩短吗啡在家兔体内的代谢时间。
Objective To establish a method of extracting morphine by LDHW-FFZ1-WuYH chromatography column and to discuss the effect of hydrochlorothiazide on the metabolic kinetics of morphine in poisoned rabbits. Methods Six rabbits were randomly divided into experimental group (morphine group+hydrochlorothiazide) and control group (morphine),every group had three rabbits.Rabbits in experimental group was extracted and injected with morphine hydrochloride saline solution through ear vein to observe the vital signs of breathing and heartbeat in rabbits.After30 minutes,hydrochlorothiazide saline was orally administered.After30 minutes,1,2,3,4,5,6,7 and 8 hours were taken from the common carotid artery for cryopreservation.Control group was only injected hydrochloride saline solution.The vital signs of respiration and heartbeat of rabbits were observed by intravenous injection through ear margin.After30 minutes,1 mL blood was taken from common carotid artery for cryopreservation at1,2,3,4,5,6,7 and 8 h,respectively.LDHW-FFZ1-WuYH column was used for pretreatment,gas chromatography (GC/NPD) was used to quantitatively detect morphine content. Results The recovery rate of LDHW-FFZ1-WuYH was high.Morphine levels of control group at3,4,5,8 h were significantly higher than that of experimental group ( P<0.05). Conclusion The method of extracting morphine by LDHW-FFZ1-WuYH column has been established in this study.This method has the advantages of low cost,convenience and rapidity,high recovery rate and low impurity content.It can be applied to the pretreatment of the samples of death cases caused by morphine poisoning.It can be seen that hydrochlorothiazide can accelerate the metabolic rate of morphine,and shorten the metabolic time of morphine in the rabbits.
作者
王勇
吴玉红
卜娟
殷豪健
黄子夏
WANG Yong;WU Yuhong;BU Juan;YIN Haojian;HUANG Zixia(Department of Criminal Science and Technology,Police Academy of Chongqing,Chongqing 401331,China;Chongqing Key Laboratory of Toxic Drug Analysis,Chongqing 401331,China;Criminal Science of Grade 2015,Chongqing Police College,Chongqing 401331,China)
出处
《检验医学与临床》
CAS
2019年第18期2596-2598,共3页
Laboratory Medicine and Clinic
基金
重庆市教育委员会科学技术研究项目(KJ1715363)
重庆警察学院科研项目(JY201802)
重庆高校创新团队建设计划(CXTDX01041)
关键词
层析柱
吗啡
氢氯噻嗪
毒物代谢动力学
chromatographic column
morphine
hydrochlorothiazide
toxicokinetics
