摘要
目的探讨胰岛素干预在2型糖尿病大鼠合并尿路感染中的应用效果。方法对2016年6月-2018年6月内60只清洁的近交系雌性Wistar大鼠行高糖、高脂的饲料进行饲养,随后进行25 mg/kg的链霉素(streplozoticin,STZ)注射,其配比方法为将STZ加入pH为4.2的0.1 mol/L的枸橼酸钠缓冲液中,最终将其浓度调整为0.25%。并于注射后的第4周再次进行24小时摄食量、体质量、血压、甘油三酯、胆固醇、血糖以及血浆胰岛素水平测定。同时,对大鼠行胰岛素抵抗实验,从而判断2型糖尿病大鼠建模是否成功。对建模成功的糖尿病大鼠禁水12 h处理,后行20%的乌拉坦进行腹腔注射进行尿路感染。将感染成功的小鼠随机分为两组,对照组大鼠行尿路清治疗,研究组大鼠行尿路清+胰岛素干预,连续治疗30 d后,对治疗效果进行观察,并探究胰岛素干预在2型糖尿病大鼠合并尿路感染作用机制。结果造模后,大鼠24 h摄食量、体质量、血压、甘油三酯、胆固醇、血糖、血浆胰岛素皆高于造模前(P<0.05),造模后大鼠血糖水平皆>7.8 mmol/L,所有大鼠2型糖尿病造模成功;对2型糖尿病大鼠进行尿路感染,有56例大鼠出现了尿频、尿急、排出尿液浑浊且有异味的症状。且这些大鼠的血清IL-6、IL-8分别>0.2、1.2 ng/ml;这56例糖尿病大鼠感染成功。治疗后,两组大鼠的空腹血糖(Fasting blood sugar,FBG)、总胆固醇(Total Cholesterol,TC)、甘油三酯(Triglyceride,TG)、白细胞介素-6(Interleukin-6,IL-6)、白细胞介素-8(Interleukin-8,IL-8)水平均有缓解,但研究组优于对照组(P<0.05);对照组大鼠的细胞间黏附分子-1(Intercellular adhesion molecule-1,ICAM-1)的表达含量高于研究组。结论相比于单用尿路清治疗2型糖尿病合并尿路感染大鼠,加入胰岛素干预效果更佳,效果体现在改善血糖、血脂。
OBJECTIVE To explore the effect of insulin intervention on urinary tract infection in type 2 diabetic rats. METHODS Totally 60 clean inbred line female Wistar rats were fed with high sugar, high fat feed from Jun. 2016 to Jun. 2018, and then injected with 25 mg/kg of STZ, which was prepared by adding STZ to 0.1 mol/L sodium citrate buffer(pH 4.2) and finally adjusting the concentration to 0.25%. The 24-hour intake, weight, blood pressure, triglycerides, cholesterol, blood glucose and plasma insulin levels were measured again at the fourth week after the injection. At the same time, insulin resistance experiments were conducted in the rats to determine whether the model of type 2 diabetic rats was successfully constructed. After 12 h treatment with water incontinence in the successfully modeled diabetic rats, 20% uratan was given via intraperitoneal injection for urinary tract infection. Infected mice were randomly divided into two groups, the control group rats were treated with "Niaoluqing", whereas the study group rats were treated with "Niaoluqing" and insulin intervention. After 30 d of continuous treatment, the treatment effects were observed, and the mechanis of insulin intervention in type 2 diabetic rats combined with urinary tract infection was investigated. RESULTS After model building, 24 h rat food intake, body weight, blood pressure, triglyceride, cholesterol, blood sugar, plasma insulin were significantly higher than before model building(P<0.05). After model building, the blood glucose levels in the rats were all >7.8 mmol/L, suggesting successful building of type 2 diabetes model in all the rats. In the 56 cases of urinary tract infection in type 2 diabetic rats, urinary frequency, urgency, turbidity and odor were observed. The serum interleukin-6(IL-6) and interleukin-8(IL-8) of these rats were greater than 0.2 and 1.2 ng/ml, respectively. The 56 diabetic rats were successfully infected. After treatment, the fasting blood glucose(FBG), total cholesterol(TC), triglyceride(TG), Il-6 and IL-8 levels in both groups of rats were improved, and the study group was significantly better than the control group(P<0.05). The expression level of intercellular adhesion molecule-1(ICAM-1) in the control group was significantly higher than that in the study group. CONCLUSION Compared with the treatment of type 2 diabetes mellitus with urinary tract infection in rats with "Niaoluqing" alone, combined use of insulin intervention achieves better results, and the effect is reflected in the improved blood sugar and blood lipid.
作者
尹进波
周宗爱
汤智越
金家温
王新红
方卫
陈年由
YIN Jin-bo;ZHOU Zong-ai;TANG Zhi-yue;JIN Jia-wen;WANG Xin-hong;FANG Wei;CHEN Nian-you(Cangnan Hospital Affiliated to Wenzhou Medical University,Wenzhou,Zhejiang 325800,China)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2019年第16期2454-2458,共5页
Chinese Journal of Nosocomiology
基金
浙江省温州市卫计委面上项目(JX6B1022)
关键词
2型糖尿病模型
尿路感染
胰岛素
尿路清
IL-6
IL-8
Type 2 diabetes model
Urinary tract infection
Insulin
Niaoluqing
Interleukin-6,Interleukin-8
