摘要
为研究宁前胡内生真菌Fusariumtricinctum次生代谢产物,采用液体发酵法发酵内生真菌,利用硅胶、凝胶、MPLC、制备液相等多种方法分离获得13个单体化合物。利用波谱方法分别鉴定为cyclo-(L-pro-L-pro)(1)、cyclo-(S-pro-S-leu)(2)、cyclo-(L-phe-L-phe)(3)、cyclo-(D-pro-L-phe)(4)、cyclo-(L-pro-L-phe)(5)、cyclo-(D-pro-L-leu)(6)、cyclo-(S-pro-S-leu)(7)、cyclo-[D-(4-hydroxyprolinyl)]-(L)-leucine(8)、cyclo-[L-(4-hydroxyprolinyl)]-(L)-leucine(9)、cyclo-(trans-4-hydroxy-L-prolyl-L-phenylalanine)(10)、cyclo-(D-cis-hyp-L-phe)(11)、苯甲酸苄酯(12)和苯乙酸(13),所有化合物均首次从内生真菌F.tricinctum中分离得到。体外抗肿瘤活性显示,化合物4、6、7对CAR、CAL27、SCC-4、SCC-9、HSC-3五种口腔癌肿瘤细胞有一定的细胞毒活性。
To study the secondary metabolites of endophytic fungus Fusarium tricinctum from Ningguo Peucedanum praeruptorum Dunn.Compounds were isolated from the EtOAc extract by chromatography technology and their structures were elucidated on the basis of comprehensive spectroscopic analysis.Thirteen compounds were isolated and their structures were identified as cyclo-( L -pro- L -pro)( 1 ),cyclo-( S -pro- S -leu)( 2 ),cyclo-( L -phe- L -phe)( 3),cyclo-( D -pro- L -phe)(4),cyclo-( L -pro- L -phe)(5),cyclo-( D -pro- L -leu)(6),cyclo-( S -pro- S -leu)(7 ),cyclo-[ D -(4-hydroxyprolinyl)]-( L )-leucine ( 8 ),cyclo-[ L -(4-hydroxyprolinyl)]-( L )-leucine (9),cyclo-( trans -4-hydroxy- L -prolyl- L -phenylalanine)(10),cyclo-( D -cis-hyp- L -phe)( 11),benzyl benzoate (12),phenylacetic acid (13 ).All these compounds were isolated from Fusarium tricinctum for the first time.The cytotoxicity assay showed that compounds 4,6 and 7 had moderate cytotoxic activities against CAR,CAL27,SCC-4,SCC-9,and HSC-3.
作者
刘丛彬
刘海涛
杨家欣
刘劲松
王刚
王国凯
LIU Cong-bin;LIU Hai-tao;YANG Jai-sing;LIU Jin-song;WANG Gang;WANG Guo-kai(School of Pharmacy,Anhui University of Chinese Medicine & Anhui Engineering Research Center of Modernized Chinese Material,Hefei 230012,China;Department of Medical Research,China Medical University Hospital,China Medical University,Taichung 40447,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2019年第9期1580-1584,共5页
Natural Product Research and Development
基金
安徽省高校优秀青年人才支持计划重点项目(gxyqZD 2019035)
安徽省高校科研创新平台团队-中药饮片产地加工与炮制一体化关键技术研究创新团队(皖教秘科[2015]49号)
