摘要
建立了QuECHERS结合UPLC-MS/MS快速测定鳜体内孔雀石绿(MG)及其代谢物残留量的方法,该方法在0.1~100.0 ng/mL质量浓度范围内线性关系良好,相关系数 r 大于0.999 8, MG和隐色孔雀石绿(LMG)在鳜肌肉中的检出限(Limit of detection, LOD)和定量限(Limit of quantitation, LOQ)分别为0.10和0.30 μg/kg。在添加水平分别为0.30、1.50和5.00 μg/kg条件下,鳜肌肉中MG和LMG平均回收率范围分别为(96.40±5.34)%^(104.00±1.75)%和(92.40 ± 2.54 )%^(107.00±8.42)%,精密度范围分别为1.68%~5.54%和2.75%~7.87%,回收率和精密度均符合残留检测要求。采用本研究建立的残留分析方法研究了阳性养殖环境中鳜体内MG和LMG残留消除规律,结果表明,阳性养殖环境中[沉积物中MG和LMG残留量为(233.48±17.07)μg/kg和(73.38±5.98)μg/kg],鳜体内MG残留以LMG为主,其残留含量均在10.0 μg/kg以下,LMG呈现先增加后降低的趋势且消除较慢,10 d时达到最高含量(9.94 μg/kg),随后呈波动式下降,直至240 d时未检出。推断若阳性养殖环境中残留MG或LMG则会造成鳜体内LMG长时间残留,易对鳜食用安全性造成隐患。为了保障鳜的食用安全性,建议对鳜养殖环境中MG和LMG残留量进行测定,确保养殖环境中无MG和LMG残留,并适当延长养殖周期。
A rapid detection method was established for measuring the malachite green and its metabolite residues in mandarin fish. The method had a good linear relationship within the concentration range of 0.1-100.0 ng/mL, and the correlation coefficient was higher than 0.999 8. The limit of detection (LOD) and the limit of quantitation (LOQ) of malachite green (MG) and leucomalachite Green (LMG) in the muscle of siniperca chuatsi were 0.10 μg/kg and 0.30 μg/kg, respectively. The average recoveries of MG and LMG at the concentration of 0.30, 1.5 and 5.0 μg/kg were (96.40±5.34)%-(104.00±1.75)% and (92.40±2.54)%-(107.00±8.42)%, respectively. The precision of MG and LMG were 1.68%-5.54% and 2.75%-7.87%, respectively. The recovery and precision were met the requirement of residue detection. The elimination regularity of MG and LMG in muscle of the mandarin fish in the positive breeding environment was studied using the established method. The results showed that in the positive breeding environment (the content of MG and LMG in sediments were (233.48±17.07)μg/kg and (73.38 ±5.98)μg/kg, respectively). The main residual of MG in mandarin fish was LMG at the concentration of less than 10.0 μg/kg. LMG eliminated slowly, and showed a trend of first increasing and then decreasing, with the highest concentration of 9.94 μg/kg at 10 days. Then it showed a fluctuation decline, and was not detected at 240 days. It was concluded that the MG and LMG in the positive breeding environment generated the long-term residual of LMG in muscle of siniperca chuatsi for a long time, with the potential risks to the food safety of the siniperca chuatsi . In order to guarantee the food safety, it is suggested to determine the MG and LMG residues in the breeding environment to ensure the quality of siniperca chuatsi without MG or LMG residues via extending the breeding cycle.
作者
李丽春
刘书贵
尹怡
郑光明
马丽莎
谢文平
戴晓欣
单奇
赵城
LI Lichun;LIU Shugui;YIN Yi;ZHENG Guangming;MA Lisha;XIE Wenping;DAI Xiaoxin;SHAN Qi;ZHAO Cheng(Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Aquatic Product (Guangzhou), Key Laboratory of Recreational Fisheries Research, Ministry of Agriculture, Pearl River Fisheries Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China)
出处
《中国渔业质量与标准》
2019年第5期48-54,共7页
Chinese Fishery Quality and Standards
基金
国家水产品质量安全风险评估项目(GJFP201800902)
广东省水产品质量安全专项项目(项目名称:不同养殖关键节点的鳜鱼中孔雀石绿残留消除及其风险评估研究)
