摘要
目的比较多重实时荧光定量PCR(multiplex real-time polymerase chain reaction,MRTPCR)和间接免疫荧光法(indirect immunofluorescence assay,IFA)对成人呼吸道病毒及非典型病原体检测结果。方法收集2014年1月至2017年12月间呼吸内科210例成人呼吸道感染的标本,采用MRTPCR检测8种常见呼吸道病原体,同时应用IFA检测血清8种病原体Ig M抗体,并全部进行PCR及测序分析,比较两种方法的特异性及敏感性,评估MRT-PCR的临床应用价值。结果 210例下呼吸道感染标本经MRT-PCR和IFA检测,阳性率分别为58.57%和38.10%,混合感染率分别为7.62%和4.76%。两种方法的灵敏度分别为94.74%和35.96%,特异度分别为84.38%和59.38%。灵敏度和特异度差异有统计学意义(P<0.05)。结论与IFA相比,MRT-PCR灵敏度、特异度好,检测性能优于IFA。
Objective To compare the detection results of the samples from the patients with lower respiratory tract infections with multiplex real-time polymerase chain reaction( PCR) assay( MRT-PCR) and indirect immunofluorescence assay( IFA). Methods The samples of the infected lower respiratory tract were taken from 210 patients in our hospital during January 2014 to December 2017. The samples were detected by MRT-PCR and IFA,respectively,and then received sequencing analysis. Then the sensitivity and specificity of the two kinds of assays were compared. Results The positive rates of the pathogens in the 210 samples for the MRT-PCR and IFA were 58. 57% and 38. 10%,respectively,and the mixed infection rates were 7. 62% and4.76%,respectively. The sensitivities for the MRT-PCR and IFA were 94.74% and 35.96%,respectively,and the specificities for the MRT-PCR and IFA were 84. 38% and 59. 38%,respectively. There were significant differences in the sensitivities and specificities of the two kinds of assays( P< 0.05). Conclusion Compared with the IFA assay,the MRT-PCR assay has a higher sensitivity and a higher specificity in the detection of the pathogens in the lower respiratory tract infections.
作者
刘馨玉
李萌
石璞玉
陈明伟
Liu Xinyu;Li Meng;Shi Puyu;Chen Mingwei(Department of Respiratory Medicine,First Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710061,China)
出处
《中华肺部疾病杂志（电子版）》
CAS
2019年第5期559-562,共4页
Chinese Journal of Lung Diseases(Electronic Edition)
基金
国家科技重大专项课题资助(2017ZX10103004)
关键词
下呼吸道感染
呼吸道病毒
非典型病原体
间接免疫荧光法
多重实时荧光定量PCR
Lower respiratory tract infections
Respiratory virus
Atypical pathogen
Indirect immunofluorescence assay
Multiplex real-time polymerase chain reaction
