摘要
目的研究PI3K及p-Smad7的表达对骨肉瘤细胞增殖的影响。方法选取2017年1月~2018年10月解放军空军军医大学唐都医院骨科收治的60例骨肉瘤患者(骨肉瘤组织)和同期60例软骨组织(正常组织)为研究对象并进行免疫组化染色,观察两种骨组织变化和PI3K及p-Smad7在不同组织中的表达情况。PI3K抑制剂CAL-101(2.5 nm)处理骨肉瘤MG-63细胞为实验组,未处理骨肉瘤MG-63细胞为对照组,观察两组MG-63细胞增殖能力(MTT增殖实验)、克隆形成能力(克隆形成实验)、PI3K及p-Smad7的表达(qRT-PCR和Western blot检测)。结果骨肉瘤组织中PI3K及p-Smad7的表达显著高于正常组织(P<0.01)。给药处理48、72 h后,实验组的MG-63细胞增殖能力明显低于对照组(P<0.05)。给药处理48 h,继续培养15 d后,实验组的MG-63细胞克隆形成能力明显低于对照组(P<0.05)。给药处理36 h后,实验组的MG-63细胞PI3K及p-Smad7表达均明显低于对照组(P<0.05)。结论 PI3K和p-Smad7相互作用异常表达可促进骨肉瘤细胞增殖。
Objective To study the effect of PI3 K and p-Smad7 expression on the proliferation of osteosarcoma cells.Methods From January 2017 to October 2018, 60 osteosarcoma patients(osteosarcoma tissues) and 60 chondroid tissues(normal tissues) from Department of Orthopedics Tangdu Hospital, Air Force Military Medical University of PLA were selected as subjects for study and immunohistochemical staining. The changes of two kinds of bone tissues and the expression of PI3 K and p-Smad7 in different tissues were observed. Osteosarcoma MG-63 cells were treated with PI3 K inhibitor CAL-101(2.5 nm) as experimental group and untreated osteosarcoma MG-63 cells as control group. The proliferation capacity(MTT proliferation experiment), cloning ability(clonogenic experiment) and expression of PI3 K and p-Smad7(detection of qRT-PCR and Western blot) of MG-63 cells in two groups were observed. Results The expressions of PI3 K and p-Smad7 in osteosarcoma tissues were significantly higher than those in normal tissues(P<0.01).After administration of 48 and 72 hours, proliferation capacity of MG-63 cells in experimental group was significantly lower than that in control group(P<0.05). After administration of 48 hours and 15 days of continuous culture, cloning ability of MG-63 cells in experimental group was significantly lower than that in control group(P<0.05). After administration of 36 hours, expression of PI3 K and p-Smad7 in MG-63 cells in experimental group was significantly lower than that in control group(P<0.05). Conclusion Abnormal expression of PI3 K and p-Smad7 interaction can promote proliferation of osteosarcoma cells.
作者
张小平
于哲
胥云
闫康
廖博
周勇
高铜拴
ZHANG Xiaoping;YU Zhe;XU Yun;YAN Kang;LIAO Bo;ZHOU Yong;GAO Tongshuan(National Institute of Bone Oncology,Tangdu Hospital,Air Force Military Medical University of PLA,Shaanxi Province,Xi′an 710032,China)
出处
《中国医药导报》
CAS
2019年第25期7-10,15,I0003,共6页
China Medical Herald
基金
国家自然科学基金资助项目(31670940)
