摘要
目的分析吴茱萸2种不同入药形式(粉末和水煎液)中化学成分的差异。方法以70%乙醇提取物和水煎液模拟2种入药形式,优选9个代表性化合物(新绿原酸、绿原酸、隐绿原酸、芦丁、金丝桃苷、去氢吴茱萸碱、吴茱萸碱、吴茱萸次碱和吴茱萸新碱)作为含有量测定指标,建立UPLC法分析不同入药形式的成分差异。结果 70%乙醇对吴茱萸各类化合物都有较好的提取效率;水煎液对酚酸、黄酮等极性较大的化合物有较好的提取效率,其中隐绿原酸在水煎液中的含有量远高于在醇提物中;生物碱,尤其是喹诺酮类生物碱在水煎液中的转移率很低。结论中医的遣方用药有其科学内涵,应该根据具体功用、安全性等因素选择适当的入药形式。
AIM To analyze the differences of chemical constituents in two different medicinal forms(powder and decoction) of Euodiae Fructus. METHODS The 70% ethanol extract and water decoction were used to simulate the above preparations, and nine typical compounds in Euodiae Fructus were picked out as quality control indexes,including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, hyperoside, dehydrostreptamine, evodiamine, ruteacarpine and evocarpine. To establish a UPLC method for the simultaneous content determination,and to analyze the differences of chemical constituents in two different medicinal forms. RESULTS Most of the compounds in Euodiae Fructus could be easily extracted with 70% ethanol. Polar compounds had good extraction rates in water decoction such as phenolic acids, flavonoid glycosides, in which cryptochlorogenic acid showed the higher contents in water decoction. The alkaloids, especially quinolones, had lower transport rates in water decoction. CONCLUSION The formulating prescriptions and clinical use of traditional Chinese medicines have their scientific connotation. The appropriate preparations should be selected according to specific functions, safety and other factors.
作者
李斐
董嘉皓
魏飞亭
乔日发
陈海芳
杨武亮
杨明
袁金斌
LI Fei;DONG Jia-hao;WEI Fei-ting;QIAO Ri-fa;CHEN Hai-fang;YANG Wu-liang;YANG Ming;YUAN Jin-bin(Ministry of Education Key Laboratory for Modern Preparation of TCM, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China)
出处
《中成药》
CAS
CSCD
北大核心
2019年第10期2410-2414,共5页
Chinese Traditional Patent Medicine
基金
国家自然科学基金(81560648)
关键词
吴茱萸
入药形式
活性成分
水煎液
醇提液
UPLC
Euodia Fructus
medicinal forms
active ingredients
water decoction
alcohol extract
UPLC
