摘要
胆固醇是哺乳动物生长发育必需的物质,但血清胆固醇浓度过高又会引起许多疾病。乳酸菌能够调控血清中胆固醇的水平,为此关于乳酸菌降胆固醇的研究受到了广泛关注。然而,胆固醇在体内的代谢是一个多因素、多水平、多蛋白和基因参与的复杂过程,目前乳酸菌对机体胆固醇代谢的调控机理仍然不明确,多数研究只针对单个或有限几个代谢酶或蛋白质进行,缺乏系统性。本文综述了胆固醇的代谢和乳酸菌调控胆固醇代谢的物质基础,展望了差异蛋白质组学技术和聚合酶链式反应芯片技术的利用前景,分析了不同模型小鼠肝脏脂蛋白、血浆和肝脏中的差异蛋白、胆固醇代谢信号通路的相关基因,为探索乳酸菌降胆固醇的活性物质基础及调控机理提供参考。
Cholesterol is essential for the growth and development of mammalian cells, but excessively high serum cholesterol can cause many diseases. Lactic acid bacteria have received extensive attention because of their ability to regulate serum cholesterol level in recent years. Cholesterol metabolism in vivo is a complex process where multiple factors, levels, proteins and genes are involved. At present, the regulatory mechanism of lactic acid bacteria on cholesterol metabolism is unclear yet. Most studies just focus on single or a few metabolic enzymes or proteins, and lack systematicness. This paper summarizes current studies on cholesterol metabolism and the substance basis for the regulation of cholesterol metabolism by lactic acid bacteria, and it discusses the applications of differential proteomics and polymerase chain reaction array to analyze the differentially expressed proteins in liver lipoprotein, plasma and liver of different mouse models and genes involved in the cholesterol metabolism pathways, with the aim of providing evidence for exploring the active substance basis and mechanism for the cholesterol-lowering effect of lactic acid bacteria.
作者
田建军
张开屏
李权威
赵艳红
靳烨
TIAN Jianjun;ZHANG Kaiping;LI Quanwei;ZHAO Yanhong;JIN Ye(College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China;Department of Food Engineering,Inner Mongolia Business and Trade Vocational College,Hohhot 010070,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第19期334-339,共6页
Food Science
基金
国家自然科学基金地区科学基金项目(31960514
31660439)
“十三五”国家重点研发计划重点专项(2016YFE0106200)
内蒙古自治区自然科学基金面上项目(2016MS0311
2017MS0363)
内蒙古自治区科技计划项目(201701008)
内蒙古自治区高等学校科学研究项目(NJZY17445)
关键词
乳酸菌
胆固醇代谢
调控机理
lactic acid bacteria
cholesterol metabolism
regulatory mechanism
