摘要
目的研究miR-196b在宫颈癌组织中的表达水平及其与人类乳头瘤病毒(HPV)18感染的关系.方法选取2016年1月至2018年1月牡丹江医学院附属红旗医院收治的80例宫颈癌患者.利用实时定量PCR法检测所有宫颈癌组织标本及对应正常黏膜组织中miR-196b的表达情况,采用液基薄层细胞学技术检测患者HPV感染情况,并据此将80例患者分为HPV18阳性组(44例)与HPV18阴性组(36例),分析miR-196b表达水平与HPV18感染、临床病理参数及患者生存期之间的关系.采用Cox多因素回归分析宫颈癌的危险因素.结果宫颈癌组织的miR-196b表达量明显高于正常黏膜组织[(0.875±0.132)vs.(0.514±0.294)],差异有统计学意义(P<0.05);宫颈癌组织中miR-196b高表达率为76.25%(61/80),低表达率为23.75%(19/80);HPV18阳性组的miR-196b高表达率明显高于HPV阴性组(90.9%vs.58.3%),ⅡB~Ⅲ分期的miR-196b高表达率明显高于Ⅰ~ⅡA分期(91.8%vs.61.3%),差异有统计学意义(P<0.05).生存分析显示miR-196b高表达且HPV18阳性患者的生存期明显短于miR-196b低表达且HPV18阴性患者或单独miR-196b高表达患者(P<0.05);Cox多因素回归分析显示病理分期高、有淋巴转移、有远处转移、miR-196b高表达且HPV18阳性为宫颈癌的高危因素.结论 miR-196b高表达的患者往往预示其HPV18感染风险大,且患者预后结局较差、生存时间短,可通过检测miR-196b表达情况及评价HPV感染风险为临床针对性治疗提供参考.
Objective To investigate the significance of miR-196b expression in cervical cancer tissues and its correlation with HPV18 infection. Methods 80 pathological specimens of cervical cancer diagnosed in our hospital from January 2016 to January 2018 were selected as research objects. The expression of miR-196b in all cervical cancer tissue specimens and corresponding normal mucosa tissues was detected by real-time quantitative PCR. According to HPV test results(TCT), miR-196b was divided into HPV18 positive group(n=44) and HPV18 negative group(n=36), and the relationship between miR-196b expression level and HPV18 infection and clinicopathological parameters was analyzed. Results the expression of miR-196b in cervical cancer tissue was significantly higher than that of miR-196b in normal mucosa tissue [(0.875±0.132) vs.(0.514±0.294)](P<0.05).The high expression rate of miR-196b in HPV18 positive group was significantly higher than that in HPV negative group(P<0.05), while in clinical TNM stage, the high expression rate of miR-196b in ⅡB-Ⅲ stage was significantly higher than that in I-ⅡA stage(91.8% vs. 61.3%)(P<0.05). The results showed that miR-196b was highly expressed and the survival time of HPV18 positive patients was significantly lower than that of miR-196 b negative patients and HPV18 negative patients or Patients with high expression of miR-196b alone(P<0.05). Conclusions miR-196b is highly expressed in cervical cancer tissues, and the high expression of miR-196 b is closely related to lymph metastasis, tumor stage, distant metastasis and HPV18 positive. Patients with high expression of miR-196b often predict an increased risk of HPV18 infection.
作者
李会影
徐明鑫
徐明研
LI Huiying;XU Mingxin;XU Mingyan(Hongqi Hospital Affiliated of Mudanjiang Medical College, Mudanjiang 157011,China)
出处
《中国肿瘤外科杂志》
CAS
2019年第5期366-369,共4页
Chinese Journal of Surgical Oncology
基金
黑龙江省卫生健康委员会课题(2018335)
