摘要
为了探讨雌激素受体(ESR1)基因的多态性及其与鹌鹑蛋品质的关系,采用PCR-RFLP和直接测序法对中国黄羽鹌鹑、朝鲜鹌鹑、北京白羽鹌鹑3个群体的ESR1基因与鹌鹑蛋品质性状进行关联分析。结果表明:鹌鹑ESR1基因外显子1、外显子4、外显子8在北京白羽鹌鹑、中国黄羽鹌鹑、朝鲜鹌鹑3个群体中都检测到了CC、CT、TT三种基因型。外显子1在3个鹌鹑群体中均表现为CC基因型频率最高;外显子8在3个鹌鹑群体中均表现为TT基因型频率最高;外显子4在中国黄羽鹌鹑、朝鲜鹌鹑中TT基因型频率最高,分别为0.409、0.617,而北京白羽鹌鹑以CC基因型频率最高(0.667)。相关性分析结果显示,在3个检测群体中,外显子4的CC基因型和CT基因型的蛋黄高度和蛋黄指数显著高于TT基因型(P<0.05),TT基因型的蛋黄宽度显著高于CC基因型和CT基因型(P<0.05),CT基因型和TT基因型的蛋壳厚度显著高于CC基因型(P<0.05)。外显子8在蛋黄指数上,CC基因型显著高于CT基因型和TT基因型(P<0.05);CT基因型和TT基因型的蛋黄宽度显著高于CC基因型(P<0.05)。ESR1基因可以作为有价值的候选基因应用于蛋用鹌鹑的分子标记辅助选择。
In order to investigate the relationship between the polymorphism of estrogen receptor ( ESR1) gene and quail egg quality,the polymorphism of ESR1 gene was detected by PCR- RFLP in three populations,including Chinese yellow quail, Korean quail and Beijing white quail,and the correlation between the polymorphism of ESR1 gene and quail egg quality traits was analyzed.The results showed that CC,CT and TT genotypes were detected in exon1,exon 4 and exon 8 of quail ESR1 gene in Beijing white quail,Chinese yellow quail and Korean quail.Exon1 showed the highest frequency of CC genotype in all three quail populations,Exon 8 showed the highest frequency of TT genotype in all three quail populations,Exon 4 showed the highest frequency of TT genotype in Chinese yellow quail and Korean quail,0.409 and 0.617,respectively,while Beijing white quail had the highest frequency of CC genotype (0.667).The results of correlation analysis showed that the yolk height and yolk index of CC genotype and CT genotype in exon 4 were significantly higher than TT genotype ( P <0.05), the yolk width of TT genotype was significantly higher than CC genotype and CT genotype ( P <0.05),and the shell thickness of CT genotype and TT genotype was significantly higher than CC genotype ( P <0.05).In exon 8,the yolk index of CC genotype was significantly higher than that of CT genotype and TT genotype ( P <0.05),and the yolk width of TT genotype of CT genotype was significantly higher than that of CC genotype ( P <0.05).ESR1 gene can be used as a valuable candidate gene for molecular marker-assisted selection in egg quails.
作者
白俊艳
樊红灯
曹恒
庞有志
宋晓杰
蒋梦娟
付学言
卢小宁
杨帅
李新月
郝伟光
李子衡
郑飞扬
BAI Junyan;FAN Hongdeng;CAO Heng;PANG Youzhi;SONG Xiaojie;JIANG Mengjuan;FU Xueyan;LU Xiaoning;YANG Shuai;LI Xinyue;HAO Weiguang;LI Ziheng;ZHENG Feiyang(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471023,China)
出处
《浙江农业学报》
CSCD
北大核心
2019年第10期1608-1614,共7页
Acta Agriculturae Zhejiangensis
基金
河南省产学研合作项目(152107000095.0)
河南科技大学标志性科技成果培育基金(2015BZCG02)
