摘要
目的建立高效液相-柱后衍生-荧光检测法测定普瑞巴林含量的方法。方法采用Waters C18色谱柱;流动相:水-甲醇(85:15);流速:1.0 mL·min^-1;检测波长:λex334 nm,λem455 nm;柱温:35℃。结果普瑞巴林在8~80μg·mL^-1范围内与峰面积呈良好的线性关系(r=0.999 5);平均回收率99.82%,RSD=0.80%(n=9)。结论该方法简便、准确、灵敏,专属性强,可用于普瑞巴林的含量测定。
Objective To establish a HPLC method to measure the content of Pregabalin with post-column derivatization and fluorescence detection. Methods A Waters C18 column was adopted with mobile phase composed of water-methanol(85:15) at the flow rate of 1.0 mL·min^-1 and column temperaturc at 35 ℃.The fluorescence detection wavelength was 334 nm(λex)and 455 nm(λem). Results The linear range was 8-80 μg·mL^-1(r=0.999 5).The average recovery was 99.82% with RSD of 0.80%(n=9). Conclusion The method is smiple,accurate,specific and sensitive for determination of content of Pregabalin by HPLC with post-column derivatization and fluorescence detection.
作者
郭旭光
GUO Xuguang(Food Inspection and Testing Institute of Henan Province, Zhengzhou 450003, China)
出处
《医药导报》
CAS
北大核心
2019年第11期1479-1481,共3页
Herald of Medicine
关键词
普瑞巴林
含量测定
色谱法
高效液相
Pregabalin
Determination
Chromatography,high permormance liquid
