摘要
采用高效液相色谱(HPLC)-示差折光检测法和斐林试剂滴定法检测绵白糖中还原糖分的含量,分析比较二者的测定结果及可操作性。结果表明,HPLC-示差折光检测法可操作强,与斐林试剂滴定法相关系数为0.983(P<0.05),具有相关性显著;斐林试剂滴定法平行样检测RSD值为0.11%~3.1%,而HPLC-示差折光检测法平行样检测RSD值为0.06%~0.9%。斐林试剂滴定法易受干扰因素如试剂溶液颜色的影响较大,终点不易判定,而HPLC-示差折光检测法操作简单、快速,受干扰因素影响相对较小。本研究为HPLC-示差折光法的准确性提供数据论证,有助于推动HPLC-示差折光法在快速检测绵白糖还原糖分的应用,提高制糖企业日常检验分析的效率。
The analysis of the reducing sugar content in soft sugar were carried out by the methods of HPLC-refractive index detection and the Fehling reagent titration, respectively, the results and maneuverability of two methods were compared. The results showed that HPLC-refractive index detection method could be operated strongly, the correlation coefficient was 0.983 (P<0.05) between these two methods with a statistically significant correlation. The RSD value of the parallel analysis for HPLC-refractive index detection method was 0.06%~0.9%, in comparison with the value of 0.11%~3.1% by Fehling reagent titration method. According to the results, it was more sensitive to the disturbing factors such as reagent solution’s color, and the end of reaction was difficult to judge using Fehling reagent titration method. Easy operation and a fast reading with a smaller deviation for the results made it more feasible for the analysis by HPLC-refractive index detection method. The results evaluated that the reliability on the analysis for the contents of reductive sugar in soft sugar by HPLC-refractive index detection method, which promoted the efficiency of regular inspection in sugar manufactories.
作者
赵亚红
杨丽
刘东旭
刘琳
迟雅丽
王健
陈花山
赵抒娜
ZHAO Ya-hong;YANG Li;LIU Dong-xu;LIU Lin;CHI Ya-li;WANG Jian;CHEN Hua-shan;ZHAO Shu-na(COFCO Tunhe Liaoning Sugar Co. Ltd., Yingkou, Liaoning 115212;COFCO Nutrition and Health Research Institute Co. Ltd., Beijing Engineering Laboratory of Geriatric Nutrition & Foods, Beijing Key Laboratory of Nutrition & Health and Food Safety, Beijing 102209;COFCO Sugar Co. Ltd., Key Laboratory of Quality & Safety Control for Sugar Crops and Tomato, Ministry of Agriculture of the PRC, Changji, Xinjiang 831100)
出处
《甘蔗糖业》
2019年第5期58-63,共6页
Sugarcane and Canesugar
基金
西部地区博士后人才资助计划(2017M613312XB)
2016年新疆自治区高层次人才引进工程计划
特殊保障食品制造关键技术研究及新产品创制(2017YFD0400500)
