摘要
数字聚合酶链式反应(dPCR)技术在生物医疗领域具有非常重要的应用价值,为了进一步投入临床诊断和病原体检测中需要在光学检测系统的检测效率和准确性方面取得进展。针对dPCR检测需求,使用ZEMAX软件设计了无限远消色差荧光精缩物镜。镜头物方数值孔径为0.1,整体放大倍率为-0.65倍,可对Φ为34.9mm的物体进行成像,物方工作距离105.3mm,像方传递函数在70lp/mm处高于0.73,相对畸变小于0.4%;镜头设计可以同时满足大视场高分辨率的荧光多通道探测要求;基于无限远荧光显微成像技术,镜头可一次性检测位于28mm×18mm基因芯片上2万个反应通道,克服现有设备拼接成像的弊端并提高检测效率。
Digital polymerase chain reaction(dPCR) technology has very important application value in biomedical field. In order to further invest in clinical diagnosis and pathogen detection, it is necessary to make progress in the detection efficiency and accuracy of optical detection system. For the requirements of dPCR detection, an infinity achromatic fluorescence condensation objective lens was designed using ZEMAX software. The lens has a numerical aperture of 0.1 and an overall magnification of-0.65 times, and imaging range is Φ 34.9 mm. The object working distance is 105.3 mm, the image transfer function is higher than 0.73 at 70 lp/mm, and the relative distortion is less than 0.4%. The lens is designed to meet the high-resolution fluorescence multi-channel detection requirements of large fields of view. Based on infinity fluorescence microscopy imaging technology, the lens can detect 20,000 reaction channels on a 28 mm×18 mm gene chip at one time, overcoming the drawbacks of existing device mosaic imaging and improving detection efficiency.
作者
陈诚
郑继红
万新军
孙刘杰
Chen Cheng;Zheng Jihong;Wan Xinjun;Sun Liujie(School of Optical-Electrical and Computer Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Shanghai Key Lab of M odern Optical System ,University of Shanghai for Science and Technology,Shanghai 200093,China;College of Communication and A rt Design,University of Shanghai for Science and Technology,Shanghai 300093,China)
出处
《光学技术》
CAS
CSCD
北大核心
2019年第5期518-525,共8页
Optical Technique
基金
上海市科学技术委员会地方院校能力建设项目——高通量PCR基因芯片荧光检测系统研制(18060502500)
