摘要
目的研究富硒麦芽对小鼠体内抗氧化能力的影响。方法将30只健康SPF级雄性昆明小鼠按体重随机分成3组,分别为对照(基础饲料)组和0.3、1.0 mg/kg富硒麦芽组,每组10只。采用自由摄食方式进行染毒,连续饲养20 d。检测小鼠心脏、大脑、肝脏、肾脏、脾脏组织和血清中的谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活力和总抗氧化能力(T-AOC)及丙二醛(MDA)的含量。结果与对照组比较,仅1.0 mg/kg富硒麦芽组小鼠心脏、大脑、肝脏、肾脏、脾脏组织和血清中的GSH-Px、SOD活力和T-AOC较高,MDA的含量较低,除脾脏MDA含量外,差异均有统计学意义(P<0.05)。且随着饲料中含硒量的增加,小鼠心脏、大脑、肝脏、肾脏、脾脏组织和血清中的GSH-Px、SOD活力和T-AOC均呈上升趋势,而MDA的含量呈下降趋势。结论补充适量的富硒麦芽可以提高小鼠不同组织中的抗氧化能力。
Objective To study the antioxidant effects of selenium -enriched malt in mice. Methods Thirty healthy male Kunming mice were randomly divided into three groups,the control (basic diet) group,0.3 mg/kg selenium-enriched group and 1.0 mg/kg selenium-enriched group. The mice were exposed to selenium by food for 20 consecutive days. The activities of glutathione peroxidase ( GSH -Px ), superoxide dismutase (SOD), and content of total antioxidant capacity (T-AOC) and malondialdehyde( MDA) in heart,cerebral,liver,kidney, spleen and serum tissues of mice were determined. Results Compared with the controls group,the activities of the GSH-Px,SOD and T-AOC in heart,cerebral,liver,kidney, spleen and serum tissues of 1.0 mg/kg selenium -enriched group mice were higher,the level of the MDA were lower;The difference was statisticaUy significant except for the content of MDA in spleen(P<0.05). The activities of GSH-Px,S0D and T-AOC in heart, brain,liver,kidney,spleen and serum tissues of mice increased with the increase of the contents of selenium in feed,while the content of MDA decreased. Conclusion Selenium-enriched malt can improve the activity of antioxidant enzymes and total antioxidant capacity in different tissues of mice.
作者
张欢欢
张甜甜
姜迎
刘丹
蔺新英
ZHANG Huan-huan;ZHANG Tian-tian;JIANG Ying;LIU Dan;LIN Xin-ying(Department of Clinical Nutrition, Center for Reproductive Medicine,Shandong University ,Ji'nan, Shandong 250001, China)
出处
《环境与健康杂志》
CAS
北大核心
2019年第3期202-205,共4页
Journal of Environment and Health
基金
山东省国土资源厅资助项目(1212010310306)
关键词
富硒麦芽
谷胱甘肽过氧化物酶
超氧化物歧化酶
总抗氧化能力
丙二醛
Selenium-enriched malt
Glutathione peroxidase
Superoxide dismutase
Total antioxidant capacity
Malondialdehyde
