摘要
以草石蚕叶片和茎段为外植体,研究植物生长调节剂对草石蚕愈伤组织和不定芽诱导的影响,筛选愈伤组织及不定芽诱导的适宜培养基配方。结果表明,茎段在MS+NAA0.6mg·L^-1+6-BA6mg·L^-1培养基上愈伤组织诱导率和干重最高,分别为96.25%和0.027g;叶片在MS+NAA0.6mg·L^-1+6-BA6mg·L^-1培养基上愈伤组织诱导率、干重和鲜重均最大,分别为97.5%、0.647g和0.132g;在MS+NAA0.1mg·L^-1+6-BA6mg·L^-1+GA33mg·L^-1培养基上愈伤组织分化不定芽率最高,为76.67%;分化芽数最多的是MS+NAA0.1mg·L^-1+6-BA4mg·L^-1+GA32mg·L^-1培养基,其芽数为13.25个。
In order to investigate effects of plant growth regulator on callus and adventitious bud induction,we used leaves and stems as explants to select suitable medium for callus and adventitious buds induction in Stachys sieboldii Miq.The results showed that the callus induction rate and dry weight were the highest on the medium MS + NAA 0.6 mg ·L ^-1 + 6-BA 6 mg ·L ^-1 for the stem explants,which were 96.25% and 0.027 g,respectively.On the medium MS + NAA 0.6 mg ·L ^-1 + 6-BA 6 mg ·L^-1 for the leaf explants, the callus induction rate,dry weight and fresh weight were the largest,which were 97.5%,0.647 g and 0.132 g,respectively.Adventitious bud percentage was the highest on the medium MS + NAA 0.1 mg ·L ^-1 + 6-BA 6 mg ·L ^-1 + GA 3 3 mg ·L^-1 ,76.67%.The medium with the largest number of buds was MS + NAA 0.1 mg ·L ^-1 + 6-BA 4 mg ·L^-1 + GA3 2 mg ·L ^-1 ,and the number of buds was 13.25.
作者
孟祥玉
熊亚
张素勤
耿广东
MENG Xiangyu;XIONG Ya;ZHANG Suqin;GENG Guangdong(College of Agriculture,Guizhou University,Guiyang 550025,China)
出处
《种子》
北大核心
2019年第10期14-18,共5页
Seed
基金
贵州省科技支撑计划项目(黔科合支撑[2018]2330号)
贵州省科技厅重大专项计划(黔科合重大专项字[2016]3002号)
关键词
草石蚕
植物生长调节剂
愈伤组织
不定芽
诱导
Stachys siebordii Miq.
plant growth regulator
callus
adventitious bud
induction
