摘要
目的研究近红外线(IRA)、紫外线(UV)对小鼠皮肤光老化及皮肤MMP-13表达的影响,观察IRA、UV在光老化中的作用及其相互关系,探索IRA引起光老化的分子机制。方法 ICR雄性小鼠随机分为5组,即空白组、IRA组、UV组、IRA/UV组、UV/IRA组。后4组小鼠接受光照不同且顺序各异。用HE染色、免疫组化、实时定量PCR、Western blot测定MMP-13表达。结果目测及HE染色显示,较之空白组小鼠,4组实验组小鼠均出现光老化表现;与空白组比较,UV联合IRA组均能上调MMP-13蛋白和mRNA的表达(P<0.05),并且IRA/UV组比UV/IRA组上调更加显著(P<0.05)。结论①IRA可引起小鼠皮肤光老化;②IRA和UV可上调MMP-13表达,并加速光老化过程;③IRA和UV共同作用于皮肤时,作用顺序不同对皮肤老化所产生影响不同。因此,在预防小鼠皮肤皱纹形成及治疗其光老化中,防止IRA辐射,避免MMP-13形成可能发挥重要作用。
Objective To detect the expression of MMP-13 in the skin of mice treated by near-infrared(IRA) and ultraviolet(UV) so as to observe their effects on skin photoaging and their interrelation and explore molecular mechanisms in IRA-induced skin photoaging. Methods Male ICR mice were randomly divided into five groups: control group(without ray exposure), IRA group, UV group, IRA/UV group, and UV/IRA group. The mice in the latter four groups had their dorsal skin exposed to different radiated ray respectively. The levels of MMP-13 protein and mRNA in the exposed skin were detected by HE, immunohistochemical method, Western blot and real-time PCR. Results Both skin lesions by visual inspection and H&E staining results showed that mice in the other four groups had skin photoaging in the exposed skin area compared with the control group. The levels of MMP-13 protein and mRNA in the exposed skin in IRA/UV and UV/IRA groups were significantly higher than those in the control mice(P<0.05). In addition, mice in IRA/UV group showed higher levels of MMP-13 protein and mRNA than those in UV/IRA group(P<0.05). Conclusion ① IRA causes skin photoaging in mice. ② UV and IRA interact with each other, up-regulate the expression of MMP-13, and promote each other in the process of photoaging. ③ The effects of IRA and UV in combination on skin photoaging are closely related to order of exposure. Taken together, avoiding IRA exposure and the expression of MMP-13 play an important role in preventing skin wrinkle formation and treatment of photoaging in mice.
作者
刘钰泽
刘平
苏慧
杨荣丽
LIU Yu-ze;LIU Ping;SU Hui;YANG Rong-li(Department of Dermatology,The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004;Department of Dermatology,Taihe Hospital,Shiyan 442000;Department of Dermatology,Xi'an No.141 Hospital,Xi'an 710089,China)
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2019年第6期906-910,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
国家自然科学基金资助项目(No.30600764)
陕西省科学技术研究与发展计划(No.2016SF-124)
西安交通大学第二附属医院院基金重点项目[No.YJ(ZD)201307]~~
