摘要
目的探讨热休克蛋白A5(HSPA5)对雨蛙肽诱导的胰腺腺泡细胞损伤的作用。方法分别用高浓度(1×10^-5 mol/L)、低浓度(1×10^-11 mol/L)的雨蛙肽建立胰腺腺泡细胞损伤模型。分别将pcDNA3.1-HSPA5、pcDNA3.1、shHSPA5和shCon以脂质体法转染胰腺腺泡AR42J细胞,用qRT-PCR法检测细胞中HSPA5 mRNA的表达,MTT法检测细胞活力,Western blot检测细胞中HSPA5的蛋白表达,流式细胞术检测细胞凋亡。结果与对照组相比,高浓度和低浓度的雨蛙肽均能造成胰腺腺泡细胞损伤,且HSPA5在其中高表达。敲减HSPA5可加重胰腺腺泡细胞的损伤,过表达HSPA5可减轻胰腺腺泡细胞损伤,还可以逆转雨蛙肽对胰腺腺泡细胞的损伤作用。结论 HSPA5可修复雨蛙肽对胰腺腺泡细胞的损伤,这将为胰腺炎的治疗提供新方向。
Objective This paper is to investigate the effect of heat shock protein A5(HSPA5) on cerulein-induced pancreatic acinar cell injury. Methods A pancreatic acinar cell injury model was established with a high concentration(1×10^-5 mol/L) and a low concentration(1×10^-11 mol/L) of cerulein. pcDNA3.1-HSPA5, pcDNA3.1, shHSPA5, and shCon were transfected into pancreatic acinar AR42 J cells by using the liposome method. The expression of HSPA5 mRNA in cells was detected by using qRT-PCR. The cell viability was detected by using the MTT assay. The protein expression of HSPA5 was detected by using Western blot. The apoptosis was detected by using flow cytometry. Results Compared with the control group, it was found that the high concentration and low concentration of cerulein could cause pancreatic acinar cell damage, and HSPA5 was highly expressed in it. Besides, the knockdown of HSPA5 could aggravate the damage of pancreatic acinar cells. Moreover, the over-expression of HSPA5 could reduce the damage of pancreatic acinar cells, and can also reverse the damage of cerulein on pancreatic acinar cells. Furthermore, the knockdown of HSPA5 could aggravate the damage of pancreatic acinar cells. Conclusion HSPA5 can rescue the damage of pancreatic acinar cells by cerulein, which will provide a new direction for the treatment of pancreatitis.
作者
张志标
余伟
马达
刘礼军
ZHANG Zhibiao;YU Wei;MA Da;LIU Lijun(Department of Emergency, Hanchuan People's Hospital, Hanchuan 431600, China;Department of General Surgery, Hanchuan People's Hospital, Hanchuan 431600, China)
出处
《国际消化病杂志》
CAS
2019年第5期357-362,共6页
International Journal of Digestive Diseases
