摘要
将GPVH1分离株接种于 13日龄非免疫鸭胚 ,收集接毒后 3～ 7日死亡鸭胚的尿囊液。纯化病毒 ,通过PCR技术 ,从病毒基因组DNA中扩增出病毒衣壳蛋白VP3完整基因片段 ,经酶切鉴定后直接与pMD 18_T质粒载体连接 ,转化感受态大肠杆菌TG1。提取重组质粒经PCR鉴定和酶切鉴定后 ,对插入片段进行序列测定及分析。结果表明 :鹅细小病毒H1分离株VP3基因全长 16 0 5bp ,编码 5 34个氨基酸 ,与国外已发表的鹅细小病毒B株核苷酸序列同源性为 98.5 % ,氨基酸序列同源性为98.3% ,表明这二个毒株亲缘关系相近。
By Polymeraes chain reaction(PCR),VP3 gene was amplified from the DNA of goose parvovirus(H1),The PCR amplified VP3 gene was cloned into pMD 18_T vecter.The recombinant plasmid was identified with restriction endonuclease analysis and PCR,and then sequenced.The result of sequence analysis showed that the VP3 gene was 1605bp and included a complete open reading frame encoding a protein of 534 amino acids,and H1 isolate shared 98.5%and 98.3% identity with B isolate at nucleotide and amino acids level respectively.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2002年第6期418-420,415,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
黑龙江省"十五"攻关项目 (GB0 1B5 0 3_0 2 )
