摘要
目的 研究金丝桃苷 (hyperin ,Hyp)对大鼠心肌缺血再灌注诱导的细胞凋亡的保护作用。方法 用荧光显微镜和电子显微镜观察 ;原位末段标记法 (TUNEL)和流式细胞术检测等。结果 Hyp(2 5 ,5 0mg·kg- 1 )对缺血 30min后再灌注 3 5h导致大鼠心肌细胞凋亡有保护作用 ;对培养的乳大鼠心肌细胞缺氧 6h再给氧 2 4h引起的细胞凋亡 ,Hyp(0 5～ 5 0 0 μmol·L- 1 )可以减少凋亡细胞的形成。结论 金丝桃苷对缺血再灌注引起的大鼠心肌细胞损伤有保护作用 ;其作用机制可能与金丝桃苷抗心肌细胞凋亡有关。
AIM To study the protective effect of hyperin on the apoptosis in rat cardiomyocyte induced by ischemia and reperfusion injury. METHODS The apoptosis in myocardium was subjected to 30 min coronary occlusion followed by 3.5 h of reperfusion in male Sprague-Dawley rats. It was provided by 6 h of anoxia followed by 24 h of reoxygenation in the cultured neonatal rat cardiomyocytes. The profile of apoptotic myocardium was observed by phase-contrast microscope, fluorescent micrograph and terminal deoxynucleotidyl transferase mediated dUTP-digoxigenin nick end-labeling (TUNEL) method. The apoptotic number in cardiomyocytes was examined by transmission electron microscopy and flow cytometry. RESULTS In the apoptosis myocardium of ischemia/reperfusion rat, hyperin (25, 50 mg·kg~ -1) was shown to inhibit the increase of apoptotic cells of the myocardium ( P<0.05, P<0.01); hyperin was (0.5～50.0 μmol·L~ -1) also shown to significantly decrease the percentage of apoptotic cells in cultured neonatal rat cardiomyocyte. CONCLUSION Hyperin may have protective effect against cardiomyocyte ischemia/reperfusion injury. Cardiomyocyte apoptosis may be involved in the mechanism.
出处
《药学学报》
CAS
CSCD
北大核心
2002年第11期849-852,共4页
Acta Pharmaceutica Sinica
