摘要
对珍稀濒危植物蒙古扁桃进行组织培养获得再生植株。实验结果表明 ,在 MS培养基上蒙古扁桃幼苗茎尖、茎切段和叶片等外植体均可以脱分化形成愈伤组织 ,并进一步分化形成再生植株。器官的脱分化与再分化决定于培养基中的激素种类及其浓度。诱导愈伤组织形成的最适培养基为 MS+6- BA 0 .8mg/ L +NAA 0 .1 mg/ L ,芽分化诱导最适培养基为 MS+6- BA 0 .8mg/ L ,诱导生根的最适培养基是 MS+IBA 0 .5 mg/
Plantlets were regenerated from the endangered and rare plant Prunun mongolica Maxim via tissue eulture.The experiment results showed that the Prunus mongolica explants of shoot apex,shoot cutting section and leaf could be dedifferentiated to callus,and the callus could be redifferentiated to regeneration plantlet on the MS medium.The organs dedifferentiation and redifferentiation was determined by homone kinds and thier concentration in MS medium.The optimum medium of callus inducing was MS+6 BA 0.8 mg/L+NAA 0.1 mg/L,the optimum medium of inducing bud was MS+6 BA 0.8 mg/L.The optimum medium of rooting was MS+IBA 0.5 mg/L.
出处
《西北植物学报》
CAS
CSCD
2002年第6期1479-1481,共3页
Acta Botanica Boreali-Occidentalia Sinica
基金
内蒙古自治区自然科学基金资助项目 ( 980 2 0 2 )
内蒙古教育厅科研基金资助项目 ( ZD980 70 2 )
关键词
珍稀濒危植物
蒙古扁桃
组织培养
植株再生
Prunus mongolica Maxim.
endangered plant
tissue culture
plantlet regeneration
