摘要
目的 用含药血清体外观察 1 5种中、西药物对大鼠新生鼠颅骨成骨细胞增殖成熟的影响 ,比较它们的作用。方法 将所试药物口饲大鼠 ,每日 2次 ,连续 5次 ,于末次给药后 1h采血 ,分离获得含药血清 ,加入成骨细胞培养液中 ,培养 3d,以MTT法和金氏法测定光密度值 ,探讨对成骨细胞增殖和细胞外液中碱性磷酸酶活性的影响。结果 所试 1 5种药物的含药血清对成骨细胞增殖均无显著作用 ;3种西药、4种中成药能显著增强碱性磷酸酶活性 ,其作用强度分别依次为安雄、特乐定、福善美以及泰格、骨疏康、鹿茸健骨、仙灵骨葆。 6种中医古方对碱性磷酸酶活性无显著增强作用。结论 安雄胶囊 (雄激素制剂 )、特乐定片 (氟制剂 )、福善美片 (双膦酸盐制剂 )和中药泰格胶囊、骨疏康颗粒、鹿茸健骨胶囊。
Objective\ To observe the effect of serums containing fifteen traditional Chinese medicines and chemicals on multiplication and maturation of osteoblast in newborn rats and compare their effective potency. Methods\ All tested medicines were orally administered to rats twice a day for consecutive five times.One hour after the last administration,rat blood was collected and serum was isolated and obtained.Then the drug\|containing serums were added into culture medium of osteoblast,and they were cultured for three days.The multiplication of osteoblast and alkaline phosphatase (AKP) activity of extracellular fluid were assayed using MTT method and Jin's method,respectively. Results\ All fifteen drug\|containing serums of the tested medicines had no significant effect on the multiplication of osteoblast.Three chemicals and four Chinee patent medicines could evidently increase AKP activity and their effective potency ranked in the following order:Andriol>Tridin>Fosamax for chemicals,and Taige>Gushukang>Lurong Jiangu>Xianling Gubao for traditional Chinese medicines.Six ancient prescriptions of traditional Chinese medicines had no obvious effect on AKP activity. Conclusion\ The drug\|containing serums of Andriol capsule(preparation of anadrogenic hormones),Tridin tablet(fluorin preparation),Fosamax tablet(diphosphonate preparation)and traditional Chinese medicines such as Taige capsule,Gushukang granule,Lurong Jiangu capsule and Xianling Gubal capsule promote differentiation and maturation of osteoblast in vitro. \;
出处
《中国骨质疏松杂志》
CAS
CSCD
2002年第4期342-345,共4页
Chinese Journal of Osteoporosis
