摘要
目的 观察大鼠心脏移植受体经不同途径感染弓形虫及术后使用环孢素A (CyclosporinA ,CsA)对免疫功能和弓形虫感染的影响。 方法 用ELISA法检测大鼠心脏移植术后受体弓形虫特异性抗体IgM、IgG及循环抗原 ,流式细胞术测定移植术后受体的T淋巴细胞亚群的变化。 结果 移植术后使用CsA可使弓形虫感染危险度提高 ,供体携带弓形虫病原体引起的感染大于受体隐性感染的复发的发生率 ,CD8+ T淋巴细胞升高更快。感染发作时CD8+ T淋巴细胞明显升高 ,CD4+ CD8+ 比值降低或倒置。 结论 移植术后使用CsA导致免疫抑制是弓形虫感染的重要原因 ,感染发作时CD8+ 是主要的细胞毒T淋巴细胞。
Objective To evaluate the effect of Toxoplasma gondii infection on immune function of the rat recipients and onset of Toxoplasmosis after heart transplantation and its correlation with the use of Cyclosporin A(CsA). Methods ELISA was used to detect recipient's specific circulating antigen (CAg) and antibodies (IgG, IgM) after the transplantation. T lymphocyte subsets in peripheral blood were examined by using immunofluorescence stain and flow cytometry (FCM) before and after heart allograft 5,10,15,20 days in rats. Results The use of CsA increased the risk of infection by T. gondii and accelerated the increase of CD8 + T lymphocyte after the transplantation. The incidence of donor acquired T.gondii infection was higher than that of reactivated silent infection in recipients before operation. The percentage of CD8 + T lymphocyte was evidently elevated due to the onset of toxoplasmosis and the ratio CD4 +/CD8 + was reduced or inverted in the meanwhile. Conclusion The immune suppression after use of CsA was the main reason leading to an activation of the silent infection of T.gondii . CD8 + was the main cytotoxic cell elevated during the infection.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2002年第5期302-304,共3页
Chinese Journal of Parasitology and Parasitic Diseases
