摘要
目的研究甘草泻心汤对溃疡性结肠炎的影响并探讨其可能机制。方法 Wistar大鼠90只,数字随机分为正常对照组、模型组、柳氮磺胺吡啶组、甘草泻心汤灌胃大、中、小剂量组及灌肠大、中、小剂量组。2,4-二硝基氯苯和醋酸复合法建立大鼠溃疡性结肠炎模型,采用免疫组化法分析结肠粘膜组织NF-κB p65的表达,ELISA法测定血清IL-10的浓度。结果甘草泻心汤可明显减少模型大鼠组织NF-κB p65的表达,提高血清IL-10浓度。结论甘草泻心汤对溃疡性结肠炎有良好疗效,其机制可能是通过抑制NF-κB为中心的炎症通路,促进抑炎因子的分泌而实现的。
Objective To study the effect of Gancao Xiexin decoction(GXD) on ulcerative colitis(UC), and explore its mechanism. Methods 90 Wistar rats were randomly divided into normal control group, UC group, Salazosulfapyridine(SASP) group, high, middle and low dose gavage groups of GXD, and high, middle and low dose clyster groups of GXD. UC model of rats was established by using 2,4-dinitrochlorobenzene and acetic acid, the expression of NF-κB p65 was detected by immunohistochemical method,and the level of IL-10 was determined by ELISA. Results GXD could decrease the expression of NF-κB p65 in colon tissue, and increase the expression of IL-10 in serum. Conclusion GXD has significant effects on UC, and its mechanism is probably related with inhibiting the expression of NF-κB and promoting the expression of anti-inflammatory cytokines.
出处
《福建中医药大学学报》
2014年第4期39-41,共3页
Journal of Fujian College of Traditional Chinese Medicine
基金
福建省教育厅省属高校科研专项基金(JK2011025)
