摘要
目的:观察电针(EA)对脊髓损伤大鼠神经运动功能的影响,基于p38 MAPK通路探讨其作用机制。方法:63只雄性成年SD大鼠按照随机数字表法分为假手术组(Sham组)、模型组(SCI组)、电针组(SCI-EA组),每组21只。Sham组不损伤脊髓,SCI组、SCI-EA组用外力砸伤脊髓的方法造模。Sham组和SCI组不做任何干预,SCI-EA组于手术后第2 d使用电针仪(连续波、频率2 Hz、强度1 mA)进行针刺,针刺部位为T10夹脊穴,30 min/次,1次/d,每5 d休息1 d,持续针刺28 d。采用脊髓损伤行为学评分(BBB)评价脊髓损伤后运动功能变化;TUNEL法检测脊髓神经细胞凋亡;免疫组织化学SP法检测脊髓组织Caspase-3阳性细胞;Western blot检测蛋白表达情况。结果:与SCI组比较,电针干预7 d后,SCI-EA组BBB评分较SCI组明显升高,差异具有统计学意义(P<0.05);SCI-EA组脊髓组织Caspase-3阳性细胞明显降低,凋亡细胞数量明显减少,差异具有统计学意义(P<0.05);SCI-EA组p38丝裂原活化蛋白激酶(p38 MAPK)阳性表达、诱导型一氧化氮合酶(iNOS)表达明显降低,差异具有统计学意义(P<0.05)。结论:电针可抑制脊髓损伤后激活的p38 MAPK通路和iNOS途径,降低脊髓神经细胞凋亡,改善脊髓损伤大鼠神经运动功能。
Objective: To observe the effect of electroacupuncture on neuromotor function of rats with spinal cord injury, and to explore its mechanism based on p38 MAPK pathway. Methods: A total of 63 adult male SD rats were randomly divided into sham group, SCI group and SCI-EA group, with 21 cases in each group. The spinal cord was not injured in Sham group, the SCI group and SCI-EA group were established by crushing the spinal cord with external force. The sham group and SCI group did not take any intervention, At the second day after operation, SCI-EA groupwas treated by electro-acupuncture instrument(continuous wave, frequency 2 Hz, intensity 1 m A), The needling site was T10 Jiaji acupoint, 30 minutes per time, once a day, resting every 5 days, continuous treatment for 28 days. Spinal cord injury behavioral score(BBB) was used to evaluate the changes of motor function after spinal cord injury;apoptosis of spinal cord neurons was detected by TUNEL method;Caspase-3 positive cells in spinal cord tissue were detected by immunohistochemical SP method;and protein expression was detected by Western blot. Results: Compared with SCI group, BBB score of SCI-EA group was significantly higher after EA intervention for seven days, with statistically significant differences(P<0.05);Caspase-3 positive cells in spinal cord tissue of SCI-EA group were significantly lower, and the number of apoptotic cells reduced significantly, The positive expression of p38 MAPK and i NOS decreased significantly, with statistically significant differences(P<0.05). Conclusion: Electroacupuncture can inhibit the activation of p38 MAPK and i NOS pathway after spinal cord injury, reduce the apoptosis of spinal cord neurons, and improve the neuromotor function of spinal cord injury rats.
作者
陈威
吴耀持
CHEN Wei;WU Yaochi(The Affiliated Sixth People's Hospital,Shanghai Jiaotong University,Shanghai 200233,China)
出处
《康复学报》
2018年第6期37-42,共6页
Rehabilitation Medicine
基金
国家中医药管理局"十二五"重点专科建设项目(ZK0901ZJ025)
上海市科委医学引导类科技支撑项目(16401933900)
