摘要
目的 :探讨黄芪注射液治疗小鼠柯萨奇B3(CVB3)病毒性心肌炎 (VMC)的疗效及其机制。方法 :Balb/C鼠 2 4只腹腔感染柯萨奇病毒B3亲心肌细胞株后 ,随机等分为两组 :实验第 8日留取心肌和血液标本 ,分别进行心肌病理检查 ,心肌穿孔素 (Perforin ,PFP)mRNA逆转录多聚酶链反应 (RT PCR)分析及血清肌酸激酶 MB(CK MB)测定。结果 :心肌组织病理改变 :黄芪治疗组明显轻于对照组 ;心肌穿孔素RT PCR半定量 :黄芪治疗组心肌Perforin的表达明显低于对照组 ;血清CK MB含量 :黄芪治疗组明显低于对照组 ;心肌PFPmRNA表达水平与血清CK MB含量呈显著正相关。结论 :可能在病毒性心肌炎的发病中起重要的作用。黄芪注射液具有抗病毒。
Objective: To investigate the effect and mechanism of Astragalus Injection (Radix Astragali) on acute murine myocarditis caused by Coxsackievirus B 3. Methods: Acute viral myocarditis was induced in 24 Balb/c mice by injection of Coxsackievirus B 3m (CVB 3m ) intraperitoneally. Half of the mice were administered Astragalus Injection(10g/kg·d). Heart and blood samples were obtained from all mice on the 8th day after CVB 3m infection for histopathological study, detection of myocardial perforin expression by reverse transcription polymerase chain reacton(RT PCR) and measurement of serum CK MB level, respectively. Results: (1) Astragalus treated mice showed a significant reduction in myocardial lesion compared with that in untreated mice. (2) Myocardial perforin expression by semi quantitative RT PCR in Astragalus treated mice was much lower than that in controls(1.10±0.07 vs 1.31±0.12, P <0.01). (3) Serum level of CK MB was significantly low in Astragalus treated mice compared with that in controls(608.92±200.84、1338.08± 649.72, P <0.01). (4) Myocardial perforin expression level showed significant positive correlation with the changes of serum CK MB( r =0.89, P <0.01). Conclusion: Astragalus Injection has therapy effects on viral myocarditis which mechanism may be linked with perforin mediated cytotoxicity from Radix Astragalus.
出处
《中成药》
CAS
CSCD
北大核心
2002年第11期857-859,共3页
Chinese Traditional Patent Medicine
