摘要
本研究以马铃薯栽培品种"荷兰15号"、"克新1号"为试验材料,研究白糖和基因型对马铃薯试管薯诱导的影响。结果表明,添加50~110 g/L白糖有利于脱毒苗壮苗、促进试管薯结薯、提高试管薯诱导率。MS固体基本培养基+100 g/L白糖,温度(17×1)℃、光照强度2 000 Lx、光照时间10 h/d诱导效果最好,初始结薯期最短为9.67 d,诱导率78.47%、单株结薯1.21个/株、单薯重77.1 mg/个、大中薯率70.01%。研究结果表明:用食用白糖替代蔗糖,能提高试管薯诱导率和大中薯率,缩短试管薯结薯周期,降低规模化生产试管薯成本。
In present research,we used Netherlands 15 and Kexin No.1 as experimental materials to study,the effects of edible sugar and genotype on microtuber induction. The results indicated that increased concentration of edible sugar not only strengthened plantlets and more favorable to tuberization but also increased inductivity. MS solid medium containing 100 g/L edible sugar,temperature(17×1)℃,light intensity 2 000 Lx,illumination time10 h/d,induced has a better effect. initial period of tuberization 9.67 d,microtuber induction rate 78.47%,No. of tuber per plant 1.21,weight per tuber 77.1 mg,rate of big and medium 70.01%. Using edible sugar to replace sucrose,could significantly increase microtuber induction,shorten the Period of tuberization,reducing cost of microtuber production.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第8期1766-1770,共5页
Molecular Plant Breeding
基金
宁夏自然科学基金项目(NZ14269)
马铃薯新品种选育(宁夏农业育种专项)
宁夏农林科学院先导资金项目(NKYG-14-05)共同资助
关键词
马铃薯
白糖
试管薯
诱导率
单薯重
Potato,Edible sugar,Microtuber,Induction,Weight per microtuber
