摘要
为揭示苦瓜雌雄花芽基因组间的分子差异,参照BSA法原理,构建了普通苦瓜雌雄花芽的DNA池和不同性型苦瓜雌雄花芽的DNA池,从26对SSR引物和30对SRAP引物中筛选特异引物对这些基因池进行差异性扩增。分析结果发现,两种标记分别筛选得到9对SRAP特异引物和8对SSR特异引物,引物有效率为30%和30.8%。苦瓜雌花芽和雄花芽DNA序列存在差异,SSR标记在分析普通苦瓜花芽DNA池和不同性型苦瓜花芽的DNA池时,多态性比率分别是10.7%和22.5%,SRAP的多态性比率则是7.4%和24.7%。SRAP扩增的雌性花芽的特异条带数较多,SSR分析雌雄花芽却只扩增出雄花芽的特异条带。但这些条带是否与性别表达基因紧密连锁,还需利用不同苦瓜的雌雄花芽DNA进行验证。这一研究结果为揭示苦瓜性别表达的分子机理奠定了基础。
The study is projected to uncover the molecular difference between female and male flower buds' genome in bitter gourd. According to BSA principle, different DNA pools of female and male flower buds were constructed from monecious bitter gourd and bitter gourds with differential sex types. Then, differential amplifications were conducted on these DNA pools with specific primers screened from 26 pairs of SSR and 30 pairs of SRAP primers. The r Results indicated that specific primers screened included 9 pairs of SRAP and 8 pairs of SSR primers, and primer efficiency of two markers reached 30% and 30.8% separately. There were differences between female and male flower buds' DNA sequences. The polymorphic rate of SSR marker reached 10.7% and22.5% when analysis was conducted on DNA pools of flower buds of monecious bitter gourd and bitter gourds with differential sex type. Correspondingly, the polymorphic rate of SRAP marker reached 7.4% and 24.7%. SRAP marker could imply more polymorphic bands in female flower buds, whereas SSR marker only implied polymorphic bands in male flower buds. As for whether these polymorphic bands linked tightly to genes related to sex genes expression or not, further test study needs to be conducted with female and male DNA from different bitter gourd materials. This study will do some benefits to uncover molecular mechanism of sex expression in bitter gourd.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第7期1764-1771,共8页
Molecular Plant Breeding
基金
广东省科技计划项目(2012A020602068)
惠州市科技计划项目(2015B010002003
2015ZX063)共同资助
关键词
苦瓜
性别表达
性型
相关序列扩增多态性
简单重复序列
Bitter guard
Sex expression
Sex type
Sequence related amplified polymorphism
Simple sequence repeats
