摘要
本研究通过卷丹百合在不同浓度的盐胁迫下确定了盐浓度阈值,利用同源克隆和RACE技术获得了盐阈值浓度处理下卷丹百合MAPK基因的保守区(Genbank登录号:JQ437268),并对其进行生物信息学分析。同时利用了DNAman软件和Blastn序列比对在基因和蛋白角度分析了MAPK基因在盐阈值浓度处理下的表达情况。分析表明,卷丹百合盐阈值浓度为1.09 mg/m L,MAPK基因保守区长627 bp,推测编码209个氨基酸,有1个高度保守的区域(TRWYRAPE)存在于MAPK氨基酸序列中,通过NCBI上的BLAST比对分析,发现这段保守区序列为促分裂原活化蛋白激酶超家族的催化活性区。
In this study, we confirmed the salt concentration threshold through Lilium lancifolium under the stress of different concentration of salt. Utilized for homology cloning and RACE technology to obtain the MAPK gene conserved domain by the stress of salt concentration threshold and analyzed it by bioinformatics. The expression of MAPK gene in Lilium lancifolium under salt concentration threshold was analyzed with using DNAMAN and BLASTN sequence alignment to both protein and gene levels. The results showed that the salt concentration threshold was 1.09 mg/m L, MAPK gene conservation region sequence was 627 bp, which presumed to encode209 amino acids. There was a highly conserved domain(TRWYRAPE) in the amino acid sequence of MAPK.Comparative analysing with BLAST of NCBI, we found it was the catalytic activity area of mitogen activated protein kinase superfamily.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第9期2274-2283,共10页
Molecular Plant Breeding
基金
国家自然基金(31572150)
高等学校博士学科点专项科研基金课题(20112103120005)共同资助
