摘要
以香菇菌株808以及18N33和18N44为亲本,制备原生质体单核体和孢子单核体,并进行单单杂交和单双杂交,试验共设置杂交组合434对,通过显微镜镜检锁状联合及与亲本的拮抗试验获得235个杂交子。以47℃热激3 h后菌丝在PDA平板上的恢复情况作为初筛标准,71个杂交子表现出了较强的耐高温能力;以高温栽培出菇试验进行复筛,8个杂交子的综合农艺性状较优,是优良的耐高温杂交子。测定耐高温杂交子的纤维素酶活性和漆酶活性,并进行ISSR分析,结果表明,杂交子的纤维素酶活性与亲本具有显著性差异,大部分杂交子的漆酶活性与双亲或亲本之一具有显著性差异,杂交子与两亲本之间都存在着不同程度的遗传差异,是杂交子作为新菌株的有力证据。
Protoplast monokaryons and spore monokaryons were made from parent strains 808, 18N33 and18N44. In total, 434 hybrid combinations including mon-mon hybridization and di-mon hybridization were conducted, and 235 real hybrids were obtained after observing clamp connection and antagonism reaction with their parents. Hybrids were preliminary screened through the recover ability of mycelia in PDA after being treated at 47℃ for three hours, and 71 hybrids exhibited better thermo-tolerant ability than their parents. Then cultivation experiments under high temperature(25℃) were done to further validate thermo-tolerant ability of fruiting bodies,and 8 hybrids showed better comprehensive agronomy traits than their parents, so these 8 hybrids were good thermo-tolerant hybrids. Cellulase activity and laccase activity were measured for these 8 hybrids and ISSR analysis was also conducted at the same time. Cellulose activity of these 8 hybrids had significant difference with their parents, and laccase activity of most hybrids had significant difference with their parents or one of their parents. Different levels of genetic difference were existance between hybrids and parents, which could be the strong evidences to prove these 8 hybrids as new strains of Xianggu mushroom(Lentinula edodes).
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第11期3145-3153,共9页
Molecular Plant Breeding
基金
国家食用菌产业技术体系(CAR-24)资助
关键词
香菇杂交
耐高温菌株
出菇栽培
酶活性
ISSR分析
Hybridization of Xianggu mushroom(Lentinula edodes)
Thermo-tolerant strains
Cultivation
Enzyme activity
ISSR analysis
