摘要
以羊角月牙藻(Selenastrum capricornutum)为生物模型,依据Organisation for Economic Cooperation and Development(OECD)的方法,研究溴氰虫酰胺对羊角月牙藻的急性毒性。通过测定羊角月牙藻生物量、光合色素、丙二醛(MDA)含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力的变化,评价溴氰虫酰胺对羊角月牙藻的毒性效应。结果表明,溴氰虫酰胺对羊角月牙藻的72 h-ErC 50为24.15 mg·L^-1、72 h-EyC 50为20.01 mg·L^-1。随着溴氰虫酰胺浓度的增加,其对羊角月牙藻生长的抑制作用不断增强,同时抑制光合色素(叶绿素a,叶绿素b,类胡萝卜素r)的合成。溴氰虫酰胺诱导羊角月牙藻产生氧化应激反应,其中,SOD活力下降,CAT活力和MDA含量上升。
Environmental toxicology of pesticide is one of research hot topics at present.Selenastrum capricornutumwas was used as biological model to analyze acute toxicity of cyantraniliprole against Selenastrum capricornutum.S.capricornutum was exposed to cyantraniliprole at different concentrations to determine the contents of biomass,photosynthetic pigments and malonaldehyde(MDA),and the activities of superoxide dismutase(SOD)and catalase(CAT).The results showed that 72 h ErC 50 and EyC 50 of cyantraniliprole to S.capricornutum were 24.15 and 20.01mg·L^-1,respectively.Cyantraniliprole inhibited the growth of S.capricornutum and reduced the synthesis of photosynthetic pigments(chlorophyll a,chlorophyll b and carotenoid r).S.capricornutum exposed to cyantraniliprole showed an oxidative stress response,leading to a decline in the SOD activity and an increase in the CAT activity and the MDA content.
作者
徐成滨
王宇心
孔伟浩
张晓凯
刘诗颖
范咏梅
XU Chengbin;WANG Yuxin;KONG Weihao;ZHANG Xiaokai;LIU Shiyin;FAN Yongmei(Institute of Tropical Agriculture and Forestry,Hainan University/Ministry of Education Key Laboratory for Green Management of Tropical Plant Diseases and Pests,Haikou,Hainan 570228,China)
出处
《热带生物学报》
2019年第2期135-139,164,共6页
Journal of Tropical Biology
基金
国家自然科学基金地区项目(31860514)
关键词
溴氰虫酰胺
羊角月牙藻
急性毒性
光合色素
抗氧化酶活性
Cyantraniliprole
Selenastrum capricornutum
acute toxicity
photosynthetic pigments
antioxidase activity
