摘要
为了解藏鸡源致病性大肠杆菌的流行情况并摸索出适合的分离鉴定方法,试验采用细菌分离纯化培养、生化试验、16S rRNA序列鉴定的方法对从西藏林芝市西藏农牧学院临床兽医大楼散养藏鸡中采集的37份粪样中分离到的8株革兰氏阴性杆菌进行研究。结果表明:本次试验所分离到的8株革兰氏阴性杆菌为大肠杆菌,本试验分离到的8株大肠杆菌的生化特性基本保持一致,16S rRNA序列的鉴定也显示符合大肠杆菌的特性,测序比对发现分离株与产毒性大肠杆菌同源性达到99%以上。说明西藏地区林芝市藏鸡群中有大肠杆菌病分布且8株分离菌株是5类常见的致病性大肠杆菌中肠产毒性大肠杆菌的一种,具有一定的致病性,提示我们在临床中要正确用药,加强本地区的饲养管理。
In order to understand the prevalence of pathogenic Escherichia coli from Tibetan chickens and explore suitable separation and identification methods,the methods of bacterial isolation and purification culture,biochemical test and 16S rRNA sequence identification were used in this experiment to study the 37 collectted samples from the scattered Tibetan Chicken of the Clinical Veterinary Building of Tibet Agriculture and Animal Husbandry College in Linzhi of Tibet,and 8 strains of Gram-negative bacilli were isolated from fecal samples.The results showed that the 8 strains of Gram-negative bacilli isolated from this experiment were Escherichia coli.The biochemical characteristics of 8 strains of Escherichia coli isolated in this experiment were basically same,and the identification of the 16S rRNA sequence also showed that it conformed to the characteristics of Escherichia coli.The sequencing alignment showed that the isolate had a homology of more than 99%with the toxigenic Escherichia coli.This paper indicated that there was Escherichia coli distribution in Tibetan chicken flocks in Linzhi of Tibet,and 8 isolates were a common type of pathogenic Escherichia coli,and it had certain pathogenicity,which suggetted that we should use drugs correctly in the clinic to strengthen the management of the area.
作者
李美娴
卓玛
赵燕娟
王刚
吴庆侠
索朗斯珠
LI Meixian;Zhuoma;ZHAO Yanjuan;WANG Gang;WU Qingxia;Suolang sizhu(Animal Science College,Tibet Agriculture & Animal Husbandry University;Provincial Key Laboratory of Tibet Plateau Animal Epidemic Disease Research,Tibet Agriculture&Animal Husbandry University;Key Laboratory of Clinical Veterinary Medicine,Tibet Agriculture&Animal Husbandry University;Nyingchi Tibet,860000,China)
出处
《高原农业》
2019年第4期430-436,共7页
Journal of Plateau Agriculture
基金
兽医学创新创业平台建设
研究生教学案例库建设
关键词
西藏
藏鸡
大肠杆菌
分离鉴定
生化试验
16S
RRNA
Tibet
Tibetn chicken
Escherichia coli
isolation and identification
biochemi cal test
16S rRNA
