摘要
考察了具有降胆固醇乳酸菌(乳酸乳球菌L14-3、鼠李糖乳杆菌118-1、粪肠球菌M53-2、植物乳杆菌117-1、植物乳杆菌23-1)的益生特性,且初探乳酸菌降胆固醇的作用机理。通过模拟胃液、胰液进行乳酸菌的耐酸耐胆盐测定,并评价了乳酸菌对抗生素的抗性。通过胆固醇、胆盐的共沉淀作用和菌体细胞吸收两种方式去除发酵液中的胆固醇,利用邻苯二甲醛法测定胆固醇的含量,确定胆固醇的去除率。结果表明:通过共沉淀作用和菌体吸收,乳酸乳球菌L14-3和鼠李糖乳杆菌118-1分别去除胆固醇的比例最高。在pH=2.0的人工胃液中,粪肠球菌M53-2和乳酸乳球菌L14-3的活菌数显著高于其他3株菌(P<0.05)。粪肠球菌M53-2对pH 3.0的人工胃液有较好的耐受性,存活率显著高于其他4株菌(P<0.05)。在pH=8.0人工胰液中,鼠李糖乳杆菌118-1和植物乳杆菌117-1的存活率显著高于其他菌株(P<0.05)。植物乳杆菌117-1耐药性最好。因此,研究具有降胆固醇的益生菌具有广阔应用前景。
The probiotic characteristics of lactic acid bacteria(Lactococcus lactis L14-3, Lactobacillus rhamnoides 118-1, Enterococcus faecalis M53-2, Lactobacillus plantarum 117-1 and Lactobacillus plantarum 23-1) were investigated and the mechanism of the effect of lactic acid bacteria on cholesterol reduction was preliminary investigated.Acid-resistant and bile salt resistance of lactic acid bacteria was determined by simulating gastric juice and pancreatic juice, and the resistance of lactic acid bacteria to antibiotics was evaluated.Removal of cholesterol from fermentation broth by two ways: co-precipitation of cholesterol and bile salt and bacterial somatic absorption. The cholesterol content was determined by phthalaldehyde method, and the removal rate of cholesterol was determined.The results showed that L14-3 of Lactococcus lactis and Lactobacillus rhamnoides 118-1 respectively had the highest cholesterol removal rate by coprecipitation and bacterial absorption.In the pH 2.0 artificial gastric juice, the number of live bacteria of Enterococcus faecalis M53-2 and Lactococcus lactis L14-3 was significantly higher than that of the other three strains(P<0.05). Enterococcus faecalis M53-2 had better tolerance to pH 3.0 artificial gastric juice, and the survival rate was significantly higher than that of the other four strains(P<0.05).The survival rates of Lactobacillus rhamnoides 118-1 and Lactobacillus plantarum 117-1 in p H 8.0 artificial pancreatic juice were significantly higher than those of other strains(P<0.05). Lactobacillus plantarum 117-1 had the best resistance. Therefore, the study of probiotics with cholesterol lowering has a broad application prospect.
作者
付永岩
赵悦含
王毅超
高达
孙长豹
姜瞻梅
刘飞
侯俊财
FU Yongyan;ZHAO Yuehan;WANG Yichao;GAO Da;SUN Changbao;JIANG Zhangmei;LIU Fei;HOU Juncai(Key Lab of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,China)
出处
《中国乳品工业》
CAS
北大核心
2019年第7期23-27,共5页
China Dairy Industry
基金
“十三五”国家重点研发计划(2016YFD0400605)
关键词
降胆固醇
机理
疏水性
耐受性
抗药性
Cholesterol lowering
Mechanism
Hydrophobicity
Tolerance
Drug resistance
