摘要
目的通过与Xpert MTB/RIF比较,评价Xpert MTB/RIF Ultra在结核分枝杆菌及利福平耐药检测中的价值。方法选取出现肺结核症状的患者,根据是否确诊肺结核以及治疗情况分为结核检测组(CDG)和耐药结核组(DRG)。分别采用Xpert MTB/RIF和Xpert MTB/RIF Ultra检测2组患者的痰标本,以MGIT960液体培养作为参考方法,比较2种方法在结核分枝杆菌及利福平耐药检测中的敏感性和特异性。结果Xpert MTB/RIF Ultra在结核分枝杆菌检测中的敏感性(96.1%)高于Xpert MTB/RIF(85.2%),特异性相同;Xpert MTB/RIF Ultra在抗酸染色涂片阴性患者的结核病检测中敏感性(75.5%)明显高于Xpert MTB/RIF(22.6%)。结论Xpert MTB/RIF Ultra在结核病患者尤其是在抗酸染色涂片阴性患者结核病检测中的价值优于Xpert MTB/RIF。
Objective To evaluate the role of Xpert MTB/RIF Ultra in the detection of Mycobacterium tuberculosis and rifampicin resistance by comparing with Xpert MTB/RIF.Methods Patients with pulmonary tuberculosis symptoms were enrolled and classified into case detection group(CDG)and multidrug-resistance risk group(DRG).The sputum samples of the 2 groups were detected by Xpert MTB/RIF and Xpert MTB/RIF Ultra,respectively.The MGIT960 liquid culturing was used as a reference method to compare the sensitivities and specificities in the detection of Mycobacterium tuberculosis and rifampicin resistance.Results The sensitivity of Xpert MTB/RIF Ultra in the detection of Mycobacterium tuberculosis was 96.1%,which was higher than that of Xpert MTB/RIF(85.2%).The specificities were the same.Xpert MTB/RIF Ultra was sensitive in the detection of tuberculosis with negative acid-fast staining smear,and the sensitivity(75.5%)was higher than that of Xpert MTB/RIF(22.6%).Conclusions Xpert MTB/RIF Ultra is superior to Xpert MTB/RIF in the detection of tuberculosis,especially in patients with negative acid-fast staining smear tuberculosis.
作者
王伟
李静
李铮
杨晶
苑星
张国龙
陈会会
WANG Wei;LI Jing;LI Zheng;YANG Jing;YUAN Xing;ZHANG Guolong;CHEN Huihui(Department of Clinical Laboratory,Henan Chest Hospital,Zhengzhou 450000,Henan,China;Henan Provincial Center for Disease Control and Prevention,Zhengzhou 450000,Henan,China)
出处
《检验医学》
CAS
2019年第8期742-745,共4页
Laboratory Medicine
基金
中美结核病科研合作项目(DMID13-0029)
河南省科技厅攻关项目(162102410043)
河南省科技厅攻关项目(2018010038)
