摘要
旨为筛选具有高效杀松材线虫活性的拮抗细菌。采用浸测法对3株芽孢杆菌进行杀线活性测定,并对高效杀线菌株发酵滤液处理线虫后的虫体进行形态观察及该菌株培养条件和杀线物质稳定性测定。结果表明3株细菌发酵滤液处理松材线虫(Bursaphelenchus xylophilus)48 h后,线虫校正死亡率均达100%,且松材线虫在蜡样芽孢杆菌(Bacillus cereus)JK-XZ3发酵滤液4倍和8倍稀释液中处理48 h后,其校正死亡率分别达99.55%和88.32%,明显高于短小芽孢杆菌(B.pumilus)HR10和瓦雷兹芽孢杆菌(B.velezensis)YH-20;该JK-XZ3菌株发酵滤液处理过的线虫虫体断裂,内含物外溢,体壁消解;该菌株在30℃培养4 d的发酵滤液杀线活性最高,且松材线虫在发酵滤液4倍和8倍稀释液中处理48 h后,其校正死亡率均达100%;松材线虫在40℃、60℃、80℃和100℃处理后的发酵滤液8倍稀释液中处理48 h后,其校正死亡率均在84%以上;在pH为8-10的发酵滤液8倍稀释液处理48 h后,其校正死亡率均在88%以上;说明该菌株发酵滤液具有耐高温和耐碱特性。蜡样芽孢杆菌JK-XZ3是一株具有开发和应用潜力的高毒力杀线菌株。
The aim of this study is to screen bacteria with efficient nematicidal activity against Bursaphelenchus xylophilus.Immersion test was to assess the nematicidal activities of 3 Bacillus strains,then the morphological depiction on the polypides of nematodes killed by fermentation filtrate of the selected efficient strains were observed,as well as the culture condition and the stability of substances with nematicidal activity were also determined.The results revealed that the corrected mortality of nematodes was 100%after B.xylophilus were treated for 48 h with fermentation filtrate by 3 bacteria strains.The corrected mortality of nematodes was still up to 99.55%and 88.32%,respectively when they were treated for 48 h with B.cereus JK-XZ3 in fermentation filtrate diluted for 4 times and 8 times.It was significantly higher than the corrected mortality of nematodes treated with B.pumilus HR10 and B.velezensis YH-20 fermented filtrate.Moreover,the polypides of nematodes treated with the fermentation filtrate of JK-XZ3 were broken,and contents overflowed,and body wall was dissolved.The filtrate of JK-XZ3 fermented in 30℃for 4d presented the highest nematicidal activity,and the corrected mortality of nematodes,when treated for 48h with the fermentation filtrate diluted for 4 and 8 times,were both up to 100%,respectively;the corrected mortality of nematodes was more than 84%when the nematodes were treated for 48 h in the 8 times-diluted fermentation filtrate processed at 40、60、80 and 100℃;at pH of 8-10,the corrected mortality of nematodes was>88%when they were treated for 48 h in 8 times-diluted fermentation filtrate.It indicated that the fermentation filtrate of the strain demonstrated the characteristics of high temperature and alkali resistances.B.cereus JK-XZ3 is a highly virulent nematicidal strain with potentials of research and application.
作者
张婉君
吴小芹
王亚会
ZHANG Wan-jun;WU Xiao-qin;WANG Ya-hui(Co-Innovation Center for Sustainable Forestry in Southern China,College of Forestry,Nanjing Forestry University,Nanjing 210037;Jiangsu Key Laboratory for Prevention and Management of Invasive Species,Nanjing 210007)
出处
《生物技术通报》
CAS
CSCD
北大核心
2019年第7期76-82,共7页
Biotechnology Bulletin
基金
国家重点研发计划(2018YFD0600203)
江苏高校优势学科建设工程项目(PAPD)
关键词
松材线虫
蜡样芽孢杆菌
发酵滤液
杀线活性
Bursaphelenchus xylophilus
Bacillus cereus
fermentation filtrate
nematicidal activity
