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不同证型慢性非萎缩性胃炎患者唾液淀粉酶的活性、蛋白含量、比活力及其N-糖基化程度 被引量:9

Preliminary Study on the Activity, Protein Content, Specific Activity and N-glycosylation Degree of Salivary Amylase in Chronic Non-atrophic Gastritis Patients with Different Syndromes
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摘要 目的分析不同证型慢性非萎缩性胃炎患者唾液淀粉酶(sAA)的活性、蛋白含量、比活力及其N-糖基化程度。方法68例慢性非萎缩性胃炎患者按中医辨证分为脾气虚弱组(24例)、脾虚湿热组(33例)、肝胃不和组(11例),另设健康对照组(36名),采集各组酸刺激前后的唾液,检测sAA活性、蛋白含量、比活力及其N-糖基化程度,并分析sAA活性、蛋白含量的酸刺激前后比值。结果与本组酸刺激前比较,酸刺激后健康对照组sAA活性及蛋白含量均升高,sAA比活力均明显下降(P<0.05)。与健康对照组比较,酸刺激后脾气虚弱组、脾虚湿热组sAA活性比值、蛋白含量比值<1及糖基化缺失的例数均增高(P<0.05),酸刺激前脾虚湿热组sAA比活力高于健康对照组(P<0.05)。酸刺激后,健康对照组、脾虚湿热组、脾气虚弱组的sAA比活力均较酸刺激前明显下降(P<0.05),但组间比较差异无统计学意义(P>0.05)。肝胃不和组的各项分析指标与健康对照组差异无统计学意义(P>0.05)。结论慢性胃炎脾气虚弱证及脾虚湿热证sAA的活性、蛋白含量及N-糖基化程度发生异常改变,脾运化功能的强弱可在唾液蛋白质分泌中有较为客观的反映。 Objective To analyze the activity,protein content,specific activity and N-glycosylation degree of salivary alpha-amylase(sAA)for chronic non-atrophic gastritis(CNAG)patients with different syndromes.Methods Saliva samples were collected from 68 CNAG patients with three types of syndrome,including 24 cases with Pi deficiency syndrome(PDS),33 cases with dampness-heat due to Pi deficiency syndrome(DPDS)and 11 cases with disharmony between Gan and Wei syndrome(DGWS),as well as 36 cases in healthy control group(HCG)before and after citric acid stimulation.The activity,protein content,specific activity and N-glycosylation degree of sAA were detected.The ratio of sAA activity and protein content before and after acid stimulation were analyzed and compared respectively.Results Compared with pre-stimulaiton,activity and protein content of sAA were increased in HCG(P<0.05)after acid stimulation,but sAA specific activity was decreased significantly(P<0.05).Compared with HCG,the cases of patients with sAA activity ratio as well protein content ratio<1 and N-glycosylation deletion in both PDS and DPDS were increased(P<0.05),and sAA specific activity under resting stituation was increased(P<0.05)in DPDS.Compared with pre-stimulaiton,sAA specific activity was decreased(P<0.05)in HCG,PDS and DPDS,without significant difference among these three groups(P>0.05).There was no significant difference in the aforementioned salivary indicators between DGWS and HCG(P>0.05).Conclusions The activity,protein content and N-glycosylation degree of sAA from CNAG patients with PDS and DPDS were altered abnormally after acid stimulation,compared with HCG.It suggests that the function of spleen governing the transportation of body fluid and nutrition may be reflected objectively from the altered results of salivary chemical composition.
作者 林传权 王东旭 梁雪丹 杨龙 王丽辉 李茹柳 邱向红 陈蔚文 LIN Chuanquan;WANG Dong-xu;LIANG Xue-dan;YANG Long;WANG Li-hui;LI Ru-liu;QIU Xiang-hong;CHEN Wei-wen(Piwei Institute,Guangzhou University of Chinese Medicine,Guangzhou(510405)
出处 《中国中西医结合杂志》 CAS CSCD 北大核心 2019年第8期937-942,共6页 Chinese Journal of Integrated Traditional and Western Medicine
基金 国家自然科学基金资助项目(No.81403297) 广州中医药大学“薪火计划”项目(No.XH20170113),广州中医药大学优秀青年学者科研基金项目(No.2013-07),广州中医药大学“青年英才培养工程”项目(No.QNYC20140114) 教育部博士点基金新教师系列项目(No.20124425120017)
关键词 慢性非萎缩性胃炎 脾气虚弱 脾虚湿热 肝胃不和 唾液淀粉酶活性 N-糖基化 chronic non-atrophic gastritis Pi deficiency syndrome dampness-heat due to Pi deficiency syndrome disharmony between Gan and Wei syndrome salivary alpha-amylase activity N-glycosylation
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