摘要
目的 建立测定知柏地黄丸中毛蕊花糖苷含量的方法。方法 采用高效液相色谱法,色谱柱为Agilent C18柱(4.6 mm×250 mm×5 μm),流动相为乙腈-0.1%乙酸溶液(13∶87,V/V),流速为1.0 mL/min,柱温为33 ℃,检测波长为334 nm,进样量为10 μL。结果 毛蕊花糖苷检测质量浓度在5.2×10^-3~208×10^-3 mg/mL浓度范围内线性关系良好(r=0.9998);精密度、稳定性、重复性试验的RSD≤1.37%;平均加样回收率为97.40%,RSD=1.28%(n=6)。结论 该法简便、准确、灵敏度高、重复性好,适用于测定知柏地黄丸中毛蕊花糖苷的含量。
Objective To establish a method for the content determination of verbascoside in zhibai dihuang Pills by RP-HPLC.Methods HPLC method was adopted.The determination was performed on Agilent C18 column(4.6 mm×250 mm×5 m)with mobile phase consisted of acetonitrile-0.1%ethylic acid solution(13:87,V/V)at the flow rate of 1.0 ml/min.The column temperature was 33℃,and detection wavelength was set at 334 nm.The sample size was 10μl.Results The linear range of verbascoside was 5.2×10^-3~208×10^-3 mg/ml(r=0.9998)with an average recovery of 97.40%(RSD=1.28%,n=6).RSDs of precision,stability and reproducibility tests were lower than 1.21%.Conclusion The method is simple,accurate,sensitive and reproducible,which can be used for the content determination of verbascoside acid in Zhibo dihuang pills.
作者
李腾
汤莹
LI Teng;TANG Ying(Department of Pharmacy,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei Province,Wuhan 430000,China)
出处
《中国中医药现代远程教育》
2019年第16期98-100,共3页
Chinese Medicine Modern Distance Education of China
关键词
知柏地黄丸
毛蕊花糖苷
高效液相色谱法
含量测定
HPLC法
中药化学
Zhibai Dihuang pills
verbascoside
high efficiency liquid chromatography
content determination
HPLC
chemistry of Chinese materia medica
