摘要
本研究以大肠杆菌参考菌株CVCC1369(O26)、CVCC3800(O153)、CVCC1489(O157)、CVCC3739(O159)、CVCC3807(O163)为菌种制备免疫抗原并免疫家兔,获得5种大肠杆菌O抗原定型原血清,而后制备多种吸收抗原以消除5种原血清的非特异性凝集素。通过微量凝集试验的方法,确定各血清的凝集价。最终确定了5种大肠杆菌O抗原定型血清的制备方法,为进一步改进大肠杆菌O抗原定型血清的制备工艺提供了思路和依据。
Antigens were prepared by using E.coli reference strains of CVCC1369(O26)、CVCC3800(O153)、CVCC1489(O157)、CVCC3739(O159)and CVCC3807(O163)respectively.And then 5 kinds of E.coli specific anti-O-antigen raw sera were obtained from rabbits immunized with the above antigens separately.Then cross-agglutinin was eliminated by non-specific absorption antigens.Cross-agglutination was detected by reacting sera with micro-agglutination test antigens respectively.Finally methods for the preparation of five kinds of E.coli specific anti-O-antigen serum were determined.Our study provides ideas and basis for further improving the preparation process of E.coli specific anti-O-antigen serum.
作者
辛凌翔
魏财文
张媛
李建
王秀丽
彭国瑞
张一帜
彭小兵
蒋玉文
XIN Ling-xiang;WEI Cai-wen;ZHANG Yuan;LI Jian;WANG Xiu-li;PENG Guo-rui;ZHANG Yi-zhi;PENG Xiao-bing;JIANG Yu-wen(China Institute of Veterinary Drug Control,Beijing 100081,China)
出处
《中国兽医杂志》
CAS
北大核心
2019年第5期21-25,30,共6页
Chinese Journal of Veterinary Medicine
基金
国家重点研发计划项目(2017YFF0208600,2017YFF0208603)
