摘要
采用HLPC法,色谱柱为Agilent Eclipse-C18柱,检测波长为330 nm,流动相为甲醇-0.2%磷酸水溶液梯度洗脱,流速为1.0 mL/min,通过中药色谱指纹图谱相似度评价结合主成分分析对10批次银菊合剂样品进行研究。结果表明,标定出11个共有峰,定性了3个共有峰,指纹图谱相似度均在0.9以上,符合指纹图谱研究技术要求,影响制剂质量差异是多成分协同作用的结果。
Using HLPC,Agilent Eclipse-C18 column,detection wavelength 330 nm,mobile phase was methanol-0.2%phosphoric acid gradient elution,flow rate was 1.0 mL/min.10 batches of Yinju mixture samples were studied by similarity evaluation of chromatographic fingerprints of traditional Chinese medicine.Results showed that thirteen common peaks were calibrated and three common peaks were qualitatively identified.The similarity of fingerprints was above 0.9,which met the requirements of fingerprint technology.The synergistic effect of multi-components affected the quality of preparations.
作者
张志
吕建伟
覃开羽
吴敏
张蓓
李俊伟
王莹
冯秀芳
卢森华
ZHANG Zhi;LYU Jian-wei;QIN Kai-yu;WU Min;ZHANG Bei;LI Jun-wei;WANG Ying;FENG Xiu-fang;LU Sen-hua(Liuzhou Hospital of Traditional Chinese Medicine,Liuzhou 545001,Guangxi,China;Yulin Center for Food and Drug Control,Yulin 537000,Guangxi,China)
出处
《湖北农业科学》
2019年第16期110-114,共5页
Hubei Agricultural Sciences
基金
广西中医药大学自然科学研究项目面上项目（2015LX050）
